Dejellying solution (Zorn lab)
2% Cysteine-hydrochloride, pH 8 in 0.1xMBS, to remove the jelly coat from fertilised Xenopus eggs and embryos before they can be manipulated.
|Volume of 0.1xMBS||Cysteine||5N NaOH|
|50 mls||1g||to pH 8.0|
|100 mls||2g||to pH 8.0|
|200 mls||4g||to pH 8.0|
Dissolve the Cysteine hydrochloride (or L-Cysteine hydrochloride-1-hydrate) in ~3/4 the final volume of 0.1xMBS.
Adjust to ~ pH 8 with 5N NaOH.
Check that the pH is correct and then adjust the final volume with 0.1xMBS.
This solution will only last one day.