Dejellying solution (Zorn lab)

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Dejellying solution:

2% Cysteine-hydrochloride, pH 8 in 0.1xMBS, to remove the jelly coat from fertilised Xenopus eggs and embryos before they can be manipulated.

Volume of 0.1xMBS Cysteine 5N NaOH
50 mls 1g to pH 8.0
100 mls 2g to pH 8.0
200 mls 4g to pH 8.0

Dissolve the Cysteine hydrochloride (or L-Cysteine hydrochloride-1-hydrate) in ~3/4 the final volume of 0.1xMBS.

Adjust to ~ pH 8 with 5N NaOH.

Check that the pH is correct and then adjust the final volume with 0.1xMBS.

This solution will only last one day.