General buffers and salt solutions

From Xenbase
Jump to: navigation, search

In this section:

HEPES 1 M, pH 7.4
1M MgCl2
10x Phosphate Buffered Saline (PBS)
20x Phosphate Buffered Saline (PBS)
PBT
Proteinase K(PK), 10 mg/ml
PTw
4M NaCl, 1 liter
SDS 10%
TAE 50X
1M Tris pH 9.5


HEPES 1 M, pH 7.4

For 1Liter:

Hepes 238.31 g

Dissolve, Adjust pH and volume. Store at RT.'


1M MgCl2

For 500 ml:

Dissolve 47.606 g MgCl2 in dH2O. Adjust volume, store at RT.


10x Phosphate Buffered Saline (PBS)

10X For 4 L bottle:

NaCl 320 g
KCl 8 g Na2HPO4 57.6 g
KH2PO4 9.6 g

Dissolve in dH2O. Adjust pH to 7.4, and bring to volume. Store at RT.


20x PBS (phosphate-buffered saline)

Na2PO4 H20 - 5.12 g

"Ha2HPO4 - 23.88 g

NaCl - 204.4 g
distilled water - 800 ml

Adjust pH to 7.4 and volume to 1 liter.


PBT

PBS (phosphate buffered saline)
2 mg/ml BSA (bovine serum albumin) (Fraction 5, reagent grade)
0.01% Triton X-100


Proteinase K(PK), 10 mg/ml

Supplied as 100 mg powder, (American Bioanalytical AB00925-000100)

Add 10 ml ddH20 for conc. of 10 mg/ml, mix until dissolved. Divide into 150 µl aliquots, store in -20C


PTw

1X PBS(phosphate buffered saline)

0.1% Tween-20


4M NaCl, 1 liter

Dissolve 233.76 g NaCl in 750 ml dH2O. Adjust volume.


SDS 10%

For 1 L:

Dissolve 100 g SDS in 800 ml of dH2O. Adjust volume, store at RT.


Sodium Phosphate Buffer(1M)

71 g Na2Hpo4
4 ml H3P04
1 Liter H20 titrate to pH 7.4


TAE 50X

For 2 L:

Tris 484 g
114.2 ml Glacial Acetic acid
200 ml 0.5 M EDTA pH 8.0

Dissolve in dH2O, adjust volume. Store at RT.


1M Tris pH 9.5

For 1 L:

Dissolve 121.14 g Tris in 700 ml dH2O. Adjust pH to 9.5 with HCl Adjust volume, store at RT.