Histone H3, phosphorylated - h3f3a - mitotic cells - from upstate

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Antigen: phosporylated histone H3 (Ser10)

Xenbase Gene Page: h3f3a

Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Host Species/Isotype:

Antigen Species: human

Applications: IF, WB

Antibody Name/Catalog Number: Cat # 06-570

Supplier: Upstate/Millipore

Comments: not sentitive to bleaching, works post in situ

Contributor: Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]

Additional Information:


option


A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H3 in red to map cells in metaphase


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry