Antibodies and lectins

Antibodies

Antibody Reagent Template

Tissue-specific Antibodies

12/101 - somites, skeletal muscle marker

3G8- pronephros and otic vesicle

4A6 – pronephros

zn-12 - neuronal marker

A-D

C-cadherin - "gene name"

E-cadherin - cdh1

cleaved caspase 3 (asp175) - casp3

alpha-catenin - ctnna1

active beta-catenin - ctnnb1

beta-catenin - ctnnb1 - H-102

phospho-beta-catenin - ctnnb1

CBFβ - cbfb - blood, CNS, blood vessels

centrin 1 - cetn1 - basal bodies

collagen type II - col2a1 - ECM

collagen type II - col2a1 - ECM broad

E-L

eya1 - eya1 - developing ear

fibrillin-2 - fbn2 - secreted from endothelium

anti-FLAG M2 monoclonal antibody

flk1 - VEGFR2 - kdr - endothelium, blood

anti-green fluorescent protein (GFP)

phospho-histone H3 - h3f3a - from cell signaling techonology

phospho-histone H3 - h3f3a - mitotic cells - from upstate

hnk-1 - b3gat1 - migrating neural crest

beta1-integrin - itgb1

active JNK - mapk8

laminin - lama1

LMO2 - lmo2 - blood, presomitic mesoderm, CNS

M-S

c-myc - myc - from roche

c-myc - myc - from sigma

anti-c-myc-peroxidase conjugate - myc

PKC mu - "gene name"

aPKC - PKC zeta - prkcz

slug - snai2 - endothelial to mesenchyme transition, neural crest

phospho-smad 1/5/8 - smad1 - smad8

T-Z

troponin - heart and other muscle

troponin - cardiac troponin I - tnni3 - heart

troponin - cardiac troponin T - tnnt2 - heart

alpha-tubulin - tuba4b - microtubules

acetylated alpha-tubulin - tuba4b

gamma-tubulin - tubg1 - basal bodies, microtubule minus ends

VEGFA - vegfa - somites, lateral plate mesoderm, head, hypochord

ZO-1 - tjp1

Lectins

ECL - pronephric nephrons

LEL - all pronephric epithelia- also faint lateral line

PNA - cement gland and scattered epidermal cells