Alpha-tubulin - For labeling microtubules: Difference between revisions
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(Created page with ''''Antibody name:''' DM1A '''Raised in:''' Mouse '''Subtype:''' IgG '''Antigen:''' Microtubules from chick embryo brain '''Fixatives:''' Works well in MEMFA or Dent's '''Sen…') |
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'''Available from:''' Sigma (cat#T9026) | '''Available from:''' Sigma (cat#T9026) | ||
'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room | '''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours. | ||
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse | '''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse | ||
Latest revision as of 10:49, 21 June 2010
Antibody name: DM1A
Raised in: Mouse
Subtype: IgG
Antigen: Microtubules from chick embryo brain
Fixatives: Works well in MEMFA or Dent's
Sensitive to bleaching: Yes
Functions post in situ?: Yes
Immunoprecipitation/Western blotting:
Available as: Ascites
Available from: Sigma (cat#T9026)
Optimized conditions: We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.
Other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse