Alpha-tubulin - tuba4b - microtubules: Difference between revisions
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
'''Antigen:''' microtubules from chick embryo brain | '''Antigen:''' microtubules from chick embryo brain | ||
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=489926 tuba4b] | '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=489926 tuba4b] | ||
'''Epitope:''' | '''Epitope:''' | ||
'''Host Species/Isotype:''' mouse IgG | '''Host Species/Isotype:''' mouse IgG | ||
'''Antigen Species:''' chicken | '''Antigen Species:''' chicken | ||
'''Applications:''' | '''Applications:''' IF, WB | ||
'''Antibody Name/Catalog Number:''' DM1A [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=T6199|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC (cat#T9026)] | '''Antibody Name/Catalog Number:''' DM1A [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=T6199|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC (cat#T9026)] | ||
'''Supplier:''' Sigma | '''Supplier:''' Sigma | ||
'''Comments:''' | '''Comments:''' IF: MEMFA 1hr @ RT, post-fix Dent's use at 1:300, sensitive to bleaching, functions post in situ, WB: use at 1:1000 to 1:10,000 | ||
'''Contributor:''' Wallingford Lab | '''Contributor:''' Wallingford Lab, Zorn Lab | ||
'''Additional Information:''' other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse | '''Additional Information:''' other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse | ||
Line 30: | Line 30: | ||
IP - immunoprecipitation, | IP - immunoprecipitation, | ||
IF - immunofluorescence, | IF - immunofluorescence, | ||
IHC - immunohistochemistry, | IHC - immunohistochemistry, | ||
EMSA - electromobility shift assay, | EMSA - electromobility shift assay, | ||
ChIP - chromatin immunoprecipitation, | ChIP - chromatin immunoprecipitation, | ||
FCM - flow cytometry | FCM - flow cytometry |
Latest revision as of 10:01, 9 March 2011
Antigen: microtubules from chick embryo brain
Xenbase Gene Page: tuba4b
Epitope:
Host Species/Isotype: mouse IgG
Antigen Species: chicken
Applications: IF, WB
Antibody Name/Catalog Number: DM1A (cat#T9026)
Supplier: Sigma
Comments: IF: MEMFA 1hr @ RT, post-fix Dent's use at 1:300, sensitive to bleaching, functions post in situ, WB: use at 1:1000 to 1:10,000
Contributor: Wallingford Lab, Zorn Lab
Additional Information: other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry