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Benzidine-peroxidase staining for blood (Conlon lab) - Revision history
2024-03-28T10:08:50Z
Revision history for this page on the wiki
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imported>Virgilio: Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Benzid..."
2011-11-22T14:39:36Z
<p>Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Benzid..."</p>
<p><b>New page</b></p><div>Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot]<br />
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link to protocol document [http://www.unc.edu/~fconlon/Protocols/Benzidine%20Staining.doc]<br />
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Benzidine Staining<br />
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(Hemoglobin histochemical stain)<br />
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1) Make up 4X Benzidine stock<br />
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:100 mg Benzidine<br />
:3.125 ml HAc (Glacial)<br />
:3.125 ml H2O<br />
:store @ 4°C in the DARK<br />
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2) Fix Embryos in 1X benzidine for 5-10 minutes<br />
:500ul Benzidine/1480ul H2O<br />
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3) Add 20ul 30% H2O2<br />
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4) Stain 2-15 minutes<br />
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5) Wash several times in 12% Glacial Acetic Acid<br />
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6) Fix 1.5 h in MEMFA<br />
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7) Change MEMFA 3X<br />
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8) Wash 3-4X EtOH<br />
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9) Store @ 4°C or 20°C in EtOH</div>
imported>Virgilio