Cleaved caspase 3 (asp175) - casp3 and C-myc - myc - from sigma: Difference between pages

Revision as of 09:01, 8 March 2011

Antigen: c-myc

Epitope: aa 408-439 synthetic peptide

Host Species/Isotype: mouse monoclonal

Antigen Species: human

Applications: IF, WB

Antibody Name: anti-c-myc clone 9E10 M4439 [1]

Supplier: Sigma

Comments: IF: MEMFA 1-2 hrs @ RT, post fix Dent's, WB: use at 1:1000 to 1:10,000

Contributor: Zorn Lab

Additional Information: detects myc-tagged overexpressed proteins well

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

@@ Line 1: / Line 1: @@
-'''Antigen:'''		cleaved caspase 3
+'''Antigen:'''		c-myc
-'''Epitope:'''		large fragment (17/19 kDa) of activated caspase 3 adjacent to Asp175
+'''Epitope:'''		aa 408-439 synthetic peptide
-'''Host Species/Isotype:'''		rabbit polyclonal
+'''Host Species/Isotype:'''		mouse monoclonal
-'''Antigen Species:'''		synthetic human peptide
+'''Antigen Species:'''		human
-'''Applications:'''		IF
+'''Applications:'''		IF, WB
-'''Antibody Name:'''		#9661 [http://www.cellsignal.com/products/9661.html]
+'''Antibody Name:'''		anti-c-myc clone 9E10 M4439 [http://www.sigmaaldrich.com/catalog/ProductDetail.do?F=SPEC&N4=M4439|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&cm_sp=Baynote-_-CountryCode~US|WebLang~-1|-_-m4439]
-'''Supplier:'''		Cell Signaling Technology
+'''Supplier:'''		Sigma
-'''Comments:'''		IF: MEMFA 30 min @ RT use at 1:300
+'''Comments:'''		IF: MEMFA 1-2 hrs @ RT, post fix Dent's, WB: use at 1:1000 to 1:10,000
 '''Contributor:'''		Zorn Lab
-'''Additional Information:'''
+'''Additional Information:'''		detects myc-tagged overexpressed proteins well
 '''Reference(s):'''