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| [[File:33ffar.jpg|200px|thumb|left|FISH, Fluorescein]] | | #REDIRECT [[XB-FEAT-485072]] |
| [[File:2876.jpg|200px|thumb|left|FCIS; fluorescent and colorimetric in situ]]
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| [[File:38-doubleFISH-big.jpg|200px|thumb|right|Double FISH, Fluorescein and Cy3]]
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| [[File:frog10x-big.jpg|200px|thumb|right|FISH plus transmitted light]]
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| These pages contain protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal.
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| *FISH protocol
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| *Fluorescein tyramide synthesis
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| *Cy3 tyramide synthesis
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| *FCIS protocol
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| FISH is not as sensitive as standard BM purple in situs, so if you have a very low abundance message it may not be detectable via fluorescence. You can however do a normal purple development for your low expression gene and a fluorescent counterstain- then overlay them to generate a FCIS image. These are both pretty and very good at highlighting quite subtle overlap
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| '''Papers and other sites:'''
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| Zhou, X. and Vize, P.D. (2004). Proximo-distal specialization of epithelial transport processes within the Xenopus pronephric tubules. [http://www.ncbi.nlm.nih.gov/pubmed/15223337?dopt=Abstract| Developmental Biology 271: 322-338.]
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| [http://www.faculty.virginia.edu/davidson/fluor_insitu/fluorescent_in_situ.html| Lance Davidson's flourescent in situ methods page]
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Latest revision as of 06:00, 10 February 2010