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General buffers and salt solutions - Revision history
2024-03-28T22:51:47Z
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imported>Virgilio: Created page with "'''In this section:''' :HEPES 1 M, pH 7.4 :1M MgCl2 :10x Phosphate Buffered Saline (PBS) :20x Phosphate Buffered Saline (PBS) :PBT :Proteinase K(PK), 10 mg/ml :PTw :4M NaCl, 1 li..."
2011-12-12T20:47:59Z
<p>Created page with "'''In this section:''' :HEPES 1 M, pH 7.4 :1M MgCl2 :10x Phosphate Buffered Saline (PBS) :20x Phosphate Buffered Saline (PBS) :PBT :Proteinase K(PK), 10 mg/ml :PTw :4M NaCl, 1 li..."</p>
<p><b>New page</b></p><div>'''In this section:'''<br />
:HEPES 1 M, pH 7.4<br />
:1M MgCl2<br />
:10x Phosphate Buffered Saline (PBS)<br />
:20x Phosphate Buffered Saline (PBS)<br />
:PBT<br />
:Proteinase K(PK), 10 mg/ml<br />
:PTw<br />
:4M NaCl, 1 liter<br />
:SDS 10%<br />
:TAE 50X<br />
:1M Tris pH 9.5<br />
<br />
<br />
'''HEPES 1 M, pH 7.4'''<br />
<br />
For 1Liter:<br />
:Hepes 238.31 g<br />
<br />
Dissolve, Adjust pH and volume. Store at RT.'<br />
<br />
<br />
'''1M MgCl2'''<br />
<br />
For 500 ml:<br />
<br />
Dissolve 47.606 g MgCl2 in dH2O. Adjust volume, store at RT.<br />
<br />
<br />
'''10x Phosphate Buffered Saline (PBS)'''<br />
<br />
10X For 4 L bottle:<br />
:NaCl 320 g<br />
:KCl 8 g Na2HPO4 57.6 g<br />
:KH2PO4 9.6 g<br />
<br />
Dissolve in dH2O. Adjust pH to 7.4, and bring to volume. Store at RT.<br />
<br />
<br />
'''20x PBS (phosphate-buffered saline)'''<br />
<br />
:Na2PO4 H20 - 5.12 g<br />
"Ha2HPO4 - 23.88 g<br />
:NaCl - 204.4 g<br />
:distilled water - 800 ml<br />
<br />
Adjust pH to 7.4 and volume to 1 liter.<br />
<br />
<br />
'''PBT'''<br />
<br />
:PBS (phosphate buffered saline)<br />
:2 mg/ml BSA (bovine serum albumin) (Fraction 5, reagent grade)<br />
:0.01% Triton X-100<br />
<br />
<br />
'''Proteinase K(PK), 10 mg/ml'''<br />
<br />
Supplied as 100 mg powder, (American Bioanalytical AB00925-000100)<br />
<br />
Add 10 ml ddH20 for conc. of 10 mg/ml, mix until dissolved.<br />
Divide into 150 µl aliquots, store in -20C<br />
<br />
<br />
'''PTw'''<br />
<br />
1X PBS(phosphate buffered saline)<br />
<br />
0.1% Tween-20<br />
<br />
<br />
'''4M NaCl, 1 liter'''<br />
<br />
Dissolve 233.76 g NaCl in 750 ml dH2O. Adjust volume.<br />
<br />
<br />
'''SDS 10%'''<br />
<br />
For 1 L:<br />
<br />
Dissolve 100 g SDS in 800 ml of dH2O. Adjust volume, store at RT.<br />
<br />
<br />
'''Sodium Phosphate Buffer(1M)'''<br />
<br />
:71 g Na2Hpo4<br />
:4 ml H3P04<br />
:1 Liter H20 titrate to pH 7.4<br />
<br />
<br />
'''TAE 50X'''<br />
<br />
For 2 L:<br />
:Tris 484 g<br />
:114.2 ml Glacial Acetic acid<br />
:200 ml 0.5 M EDTA pH 8.0<br />
<br />
Dissolve in dH2O, adjust volume. Store at RT.<br />
<br />
<br />
'''1M Tris pH 9.5'''<br />
<br />
For 1 L:<br />
<br />
Dissolve 121.14 g Tris in 700 ml dH2O. Adjust pH to 9.5 with HCl Adjust volume, store at RT.</div>
imported>Virgilio