https://wiki.xenbase.org/xenwiki/index.php?title=Microinjection&feed=atom&action=historyMicroinjection - Revision history2024-03-29T09:29:12ZRevision history for this page on the wikiMediaWiki 1.40.1https://wiki.xenbase.org/xenwiki/index.php?title=Microinjection&diff=47386&oldid=previmported>Virgilio: Created page with "'''In this section:''' :Ficoll, 2.5% :2.5% Ficoll in 1/3X MR (for use with X. laevis embryos) :3% Ficoll in 1/9XMR (for use with X.tropicalis embryos) '''Ficoll, 2.5%''' 2.5..."2011-12-16T14:23:51Z<p>Created page with "'''In this section:''' :Ficoll, 2.5% :2.5% Ficoll in 1/3X MR (for use with X. laevis embryos) :3% Ficoll in 1/9XMR (for use with X.tropicalis embryos) '''Ficoll, 2.5%''' 2.5..."</p>
<p><b>New page</b></p><div>'''In this section:'''<br />
<br />
:Ficoll, 2.5%<br />
:2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)<br />
:3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)<br />
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<br />
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'''Ficoll, 2.5%'''<br />
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2.5 g ficoll/100ml DI water (usually make 500 ml)<br />
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Autoclave and store at RT.<br />
<br />
Use in place of water in dish to stabilize embryos<br />
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'''2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)'''<br />
<br />
Dissolve 2.5 g/100ml of Ficoll in 1/3X MR and autoclave(Takes long time to dissolve.)<br />
<br />
Store at RT.<br />
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<br />
'''3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)'''<br />
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Dissolve 3 g/100ml of Ficoll in 1/9X MR and autoclave(Takes long time to dissolve.)<br />
<br />
Store at RT</div>imported>Virgilio