Microinjection

From Xenbase
Revision as of 14:23, 16 December 2011 by Anonymous (talk) (Created page with "'''In this section:''' :Ficoll, 2.5% :2.5% Ficoll in 1/3X MR (for use with X. laevis embryos) :3% Ficoll in 1/9XMR (for use with X.tropicalis embryos) '''Ficoll, 2.5%''' 2.5...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

In this section:

Ficoll, 2.5%
2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)
3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)


Ficoll, 2.5%

2.5 g ficoll/100ml DI water (usually make 500 ml)

Autoclave and store at RT.

Use in place of water in dish to stabilize embryos


2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)

Dissolve 2.5 g/100ml of Ficoll in 1/3X MR and autoclave(Takes long time to dissolve.)

Store at RT.


3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)

Dissolve 3 g/100ml of Ficoll in 1/9X MR and autoclave(Takes long time to dissolve.)

Store at RT