Histone H3, phosphorylated - h3f3a - mitotic cells - from upstate and Smad 1/5/8, phosphorylated - smad1 - smad8: Difference between pages

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(Created page with "'''Antigen:''' human phospho-smad1/smad5/smad8 '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=1018192 smad1] [http://www.xenbase.org...")
 
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'''Antigen:''' phosporylated histone H3 (Ser10)
'''Antigen:''' human phospho-smad1/smad5/smad8
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a]
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=1018192 smad1] [http://www.xenbase.org/gene/showgene.do?method=display&geneId=484685 smad8]
'''Epitope:'''
'''Host Species/Isotype:''' rabbit polyclonal
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
'''Host Species/Isotype:'''
'''Antigen Species:''' human
'''Antigen Species:''' human
'''Applications:''' IF, WB
'''Applications:''' WB
 
'''Antibody Name/Catalog Number:''' [http://www.millipore.com/catalogue/item/06-570 Cat # 06-570]
'''Antibody Name/Catalog Number:''' phospho-smad1/smad5/smad8 antibody [http://www.cellsignal.com/products/9511.html #9511]
 
'''Supplier:''' Upstate/Millipore
'''Supplier:''' cell signalling
 
'''Comments:''' not sentitive to bleaching, works post in situ
'''Comments:''' works reasonably well on WB
 
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Contributor:''' Rachel Stephenson
'''Additional Information:'''
'''Additional Information:'''
'''Reference(s):'''
[[File:Ph3.jpg|option|400px]]
A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H3 in red to map cells in metaphase
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IP - immunoprecipitation,
IP - immunoprecipitation,
 
IF - immunofluorescence,
IF - immunofluorescence,
 
IHC - immunohistochemistry,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry
FCM - flow cytometry

Revision as of 15:01, 8 March 2011

Antigen: human phospho-smad1/smad5/smad8

Xenbase Gene Page: smad1 smad8

Epitope:

Host Species/Isotype: rabbit polyclonal

Antigen Species: human

Applications: WB

Antibody Name/Catalog Number: phospho-smad1/smad5/smad8 antibody #9511

Supplier: cell signalling

Comments: works reasonably well on WB

Contributor: Rachel Stephenson

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

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