Polyclonal anti-phospho-H3 - mitotic cells: Difference between revisions

Latest revision as of 18:06, 15 January 2010

Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]

Antibody name: Anti-phospho-Histone H3 (Ser10)

Raised in: rabbit

Subtype: IgG

Antigen: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Fixatives: MEMFA

Sensitive to bleaching: No

Functions post in situ?: Yes

Immunoprecipitation/Western blotting: Yes, very good, 17kD target

Available as: 1 mg/ml solution.

Available from: Upstate, Cat # 06-570 [3]

Optimized conditions: Working dilution approx 1/500

Other species: Pretty much everything from tetrahymena to humans

Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)

@@ Line 1: / Line 1: @@
-'''Anti-phospho-Histone H3 (Ser10)'''
+Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
-Generated and characterized by XXX [http://www.xenbase.org:80/community/person.do?method=display&personId=XXX]
+'''Antibody name:''' Anti-phospho-Histone H3 (Ser10)
-'''Antibody name:''' XXX
 '''Raised in:''' rabbit
@@ Line 20: / Line 18: @@
 '''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target
-'''Available as:''' 1 mg/ml solution. Working dilution approx 1/500
+'''Available as:''' 1 mg/ml solution.
 '''Available from:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]
-'''Optimized conditions:'''
+'''Optimized conditions:''' Working dilution approx 1/500
 '''Other species:''' Pretty much everything from tetrahymena to humans
+[[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)