Polyclonal anti-phospho-H3 - mitotic cells: Difference between revisions
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'''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target | '''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target | ||
'''Available as:''' 1 mg/ml solution. | '''Available as:''' 1 mg/ml solution. | ||
'''Available from:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570] | '''Available from:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570] | ||
'''Optimized conditions:''' | '''Optimized conditions:''' Working dilution approx 1/500 | ||
'''Other species:''' Pretty much everything from tetrahymena to humans | '''Other species:''' Pretty much everything from tetrahymena to humans | ||
[[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green) | [[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green) | ||
Latest revision as of 18:06, 15 January 2010
Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]
Antibody name: Anti-phospho-Histone H3 (Ser10)
Raised in: rabbit
Subtype: IgG
Antigen: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
Fixatives: MEMFA
Sensitive to bleaching: No
Functions post in situ?: Yes
Immunoprecipitation/Western blotting: Yes, very good, 17kD target
Available as: 1 mg/ml solution.
Available from: Upstate, Cat # 06-570 [3]
Optimized conditions: Working dilution approx 1/500
Other species: Pretty much everything from tetrahymena to humans
Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)