Protocols: Difference between revisions
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**[[Stukenberg Lab - tissue culture of Xenopus cell lines S3, XTC]] | **[[Stukenberg Lab - tissue culture of Xenopus cell lines S3, XTC]] | ||
**[[Stukenberg Lab - isolation of mitotic chromosomes from XTC cells]] | **[[Stukenberg Lab - isolation of mitotic chromosomes from XTC cells]] | ||
**[[ | **[[Stukenberg Lab - in-vitro kinetochore-microtubule binding assays]] | ||
**[[Immunofluorescence with biotinylated antibodies]] | **[[Immunofluorescence with biotinylated antibodies]] | ||
Revision as of 08:37, 15 November 2011
- Animal Protocols
- Purifying Polyclonal Antibodies using Affinity Columns / Chromatography
- Embryo Staining Protocols
- Xenopus Oocyte and Egg Extracts
- Crude interphase egg extract ("LSS" - low speed supernatant)
- Isolation of chromatin from egg extract and histone recovery
- Preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)
- Cycling Extract (2)
- Cycling Extract (3)
- CSF Extract (1)
- CSF Extract (2)
- Assaying H1 kinase in extracts
- Xenopus Tissue Culture