Protocols: Difference between revisions
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*'''In Situ Hybridization''' | *'''In Situ Hybridization''' | ||
**[[in situ hybridization, brief version (Harland lab)]] | **[[in situ hybridization, brief version (Harland lab)]] | ||
**[[Fluorescent In-Situs and FCIS (Vize lab)]] | |||
* | ***[[Wholemount embryo FISH / Double FISH]] | ||
**[[Wholemount embryo FISH / Double FISH]] | ***[[Flourescin Tyramide Synthesis]] | ||
**[[Flourescin Tyramide Synthesis]] | ***[[Cy3 Tyramide Synthesis]] | ||
**[[Cy3 Tyramide Synthesis]] | ***[[FCIS]] | ||
**[[FCIS]] | ***[[Chromosomal FISH-TSA]] | ||
**[[Chromosomal FISH-TSA]] | |||
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**[[Part 2: ODN Modification]] | **[[Part 2: ODN Modification]] | ||
**[[Part 3: Host Transfer Technique]] | **[[Part 3: Host Transfer Technique]] | ||
**[[Part 4:Fertilization and Development]] | **[[Part 4: Fertilization and Development]] | ||
Revision as of 09:19, 16 November 2011
- Animal Husbandry/Animal Protocols
- In Situ Hybridization
- Immunohisochemistry
- Other Embryo Staining Protocols
- Other Immuno Protocols
- Xenopus Oocyte and Egg Extracts
- crude interphase egg extract (Shechter lab)
- isolation of chromatin from egg extract and histone recovery (Shechter lab)
- preparation of cycling egg extract (Shechter lab)
- cycling extracts, mike's protocol (Stukenberg lab)
- cycling extracts, sara's protocol (Stukenberg lab)
- CSF extract, mike's protocol (Stukenberg lab)
- CSF extract, sara's protocol (Stukenberg lab)
- assaying H1 kinase in extracts (Stukenberg lab)
- Xenopus Tissue Culture
- Purifying Polyclonal Antibodies using Affinity Columns / Chromatography