Protocols and XB-ANAT-145: Difference between pages

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*'''Animal Protocols'''
=glomerus=
**[[Inducing egg laying via hCG injection]]
This is the community wiki page for the ''glomerus'' please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase
 
 
*'''Purifying Polyclonal Antibodies using Affinity Columns / Chromatography'''
**[[Making an Affinity Column]]
**[[Running an Affinity Column]]
 
 
*'''[[Oocyte Transfer Technique]]'''
**[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]]
**[[Part 2: ODN Modification]]
**[[Part 3: Host Transfer Technique]]
**[[Part 4:Fertilization and Development]]
 
 
*'''[[Fluorescent In-Situs and FCIS]]'''
**[[Wholemount embryo FISH / Double FISH]]
**[[Flourescin Tyramide Synthesis]]
**[[Cy3 Tyramide Synthesis]]
**[[FCIS]]
**[[Chromosomal FISH-TSA]]
 
 
*'''Embryo Staining Protocols'''
**[[Apoptosis via TUNEL]]
**[[Benzidine-peroxidase staining for blood]]
**[[In situ hybridization]]
**[[Wholemount antibody staining]]
 
 
*'''Xenopus Oocyte and Egg Extracts'''
**[[Crude interphase egg extract ("LSS" - low speed supernatant)]]
**[[Isolation of chromatin from egg extract and histone recovery]]
**[[Preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)]]
**[[Cycling Extract (2)]]
**[[Cycling Extract (3)]]
**[[CSF Extract (1)]]
**[[CSF Extract (2)]]
**[[Assaying H1 kinase in extracts]]
 
 
*'''Xenopus Tissue Culture'''
**[[Tissue culture of Xenopus cell lines S3, XTC]]
**[[Isolation of mitotic chromosomes from XTC cells]]
**[[In-vitro kinetochore-microtubule binding assays]]
**[[Immunofluorescence with biotinylated antibodies]]
 
 
*'''Other Protocols'''
**[[Immunoprecipitation]]
**[[Stukenberg Lab - silver staining protein gels]]

Latest revision as of 06:00, 10 July 2010

glomerus

This is the community wiki page for the glomerus please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase