Protocols and XB-ANAT-145: Difference between pages

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*'''Animal Protocols'''
=glomerus=
**[[Inducing egg laying via hCG injection]]
This is the community wiki page for the ''glomerus'' please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase
 
 
*'''Purifying Polyclonal Antibodies using Affinity Columns / Chromatography'''
**[[Making an Affinity Column]]
**[[Running an Affinity Column]]
 
 
*'''[[Heasman Lab - Oocyte Transfer Technique]]'''
**[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]]
**[[Part 2: ODN Modification]]
**[[Part 3: Host Transfer Technique]]
**[[Part 4:Fertilization and Development]]
 
 
*'''[[Vize Lab - Fluorescent In-Situs and FCIS]]'''
**[[Wholemount embryo FISH / Double FISH]]
**[[Flourescin Tyramide Synthesis]]
**[[Cy3 Tyramide Synthesis]]
**[[FCIS]]
**[[Chromosomal FISH-TSA]]
 
 
*'''Embryo Staining Protocols'''
**[[Apoptosis via TUNEL]]
**[[Benzidine-peroxidase staining for blood]]
**[[Harland Lab - in situ hybridization (brief version)]]
**[[Wholemount antibody staining]]
 
 
*'''Xenopus Oocyte and Egg Extracts'''
**[[Shechter Lab - crude interphase egg extract ("LSS" - low speed supernatant)]]
**[[Shechter Lab - isolation of chromatin from egg extract and histone recovery]]
**[[Shechter Lab - preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)]]
**[[Stukenberg Lab - Mike's cycling extracts]]
**[[Stukenberg Lab - Sara's cycling extracts]]
**[[Stukenberg Lab - Mike's CSF extract]]
**[[Stukenberg Lab - Sara's CSF extract]]
**[[Stukenberg Lab - assaying H1 kinase in extracts]]
 
 
*'''Xenopus Tissue Culture'''
**[[Stukenberg Lab - tissue culture of Xenopus cell lines S3, XTC]]
**[[Stukenberg Lab - isolation of mitotic chromosomes from XTC cells]]
**[[Stukenberg Lab - in-vitro kinetochore-microtubule binding assays]]
**[[Stukenberg Lab - immunofluorescence with biotinylated antibodies]]
 
 
*'''Other Protocols'''
**[[Stukenberg Lab - immunoprecipitation]]
**[[Stukenberg Lab - silver staining protein gels]]

Latest revision as of 06:00, 10 July 2010

glomerus

This is the community wiki page for the glomerus please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase