Protocols and XB-ANAT-145: Difference between pages

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*'''Animal Husbandry/Animal Protocols'''
=glomerus=
**[[inducing egg laying via hCG injection (Vize lab)]]
This is the community wiki page for the ''glomerus'' please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase
 
 
*'''In Situ Hybridization'''
**[[in situ hybridization, brief version (Harland lab)]]
 
*'''[[Fluorescent In-Situs and FCIS (Vize lab)]]'''
**[[Wholemount embryo FISH / Double FISH]]
**[[Flourescin Tyramide Synthesis]]
**[[Cy3 Tyramide Synthesis]]
**[[FCIS]]
**[[Chromosomal FISH-TSA]]
 
 
*'''Immunohisochemistry'''
**[[wholemount antibody staining (CSH Protocols) (Wallingford lab)]]
 
 
*'''Other Embryo Staining Protocols'''
**[[benzidine-peroxidase staining for blood (Thomsen lab)]]
**[[apoptosis via TUNEL (Veenstra lab)]]
 
 
*'''Other Immuno Protocols'''
**[[immunoprecipitation (Stukenberg lab)]]
**[[silver staining protein gels (Stukenberg lab)]]
 
 
*'''[[Oocyte Transfer Technique (Heasman/Wylie labs)]]'''
**[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]]
**[[Part 2: ODN Modification]]
**[[Part 3: Host Transfer Technique]]
**[[Part 4:Fertilization and Development]]
 
 
*'''Xenopus Oocyte and Egg Extracts'''
**[[Shechter Lab - crude interphase egg extract ("LSS" - low speed supernatant)]]
**[[Shechter Lab - isolation of chromatin from egg extract and histone recovery]]
**[[Shechter Lab - preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)]]
**[[Stukenberg Lab - Mike's cycling extracts]]
**[[Stukenberg Lab - Sara's cycling extracts]]
**[[Stukenberg Lab - Mike's CSF extract]]
**[[Stukenberg Lab - Sara's CSF extract]]
**[[Stukenberg Lab - assaying H1 kinase in extracts]]
 
 
*'''Xenopus Tissue Culture'''
**[[Stukenberg Lab - tissue culture of Xenopus cell lines S3, XTC]]
**[[Stukenberg Lab - isolation of mitotic chromosomes from XTC cells]]
**[[Stukenberg Lab - in-vitro kinetochore-microtubule binding assays]]
**[[Stukenberg Lab - immunofluorescence with biotinylated antibodies]]
 
 
*'''Purifying Polyclonal Antibodies using Affinity Columns / Chromatography'''
**[[Making an Affinity Column]]
**[[Running an Affinity Column]]

Latest revision as of 06:00, 10 July 2010

glomerus

This is the community wiki page for the glomerus please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase