Protocols and XB-ANAT-145: Difference between pages

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*'''Books for Xenopus Research and Protocols'''
=glomerus=  
**'''Normal Table of Xenopus Laevis (Daudin)''', Nieuwkoop P D, Faber J. (Third edition), 1994 [http://www.xenbase.org/literature/book.do?method=display&bookId=1]
This is the community wiki page for the ''glomerus'' please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase
***a systematic and chronological description of Xenopus laevis development
**'''Early Development of Xenopus laevis: A Laboratory Manual''', Sive, Hazel, Grainger, Robert M, Harland, Richard M. 2000 [http://www.xenbase.org/literature/book.do?method=display&bookId=22]
***comprehensive collection of protocols for the study of early development in Xenopus embryos
**'''Xenopus Protocols: Cell Biology and Signal Transduction''', Liu X J. (First edition), 2006 [http://www.xenbase.org/literature/book.do?method=display&bookId=206]
***step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
**'''Color Atlas of Xenopus laevis Histology''', Wiechmann, Allan F , Wirsig-Wiechmann, Celeste R, 2003 [http://www.xenbase.org/literature/book.do?method=display&bookId=82]
***central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis
**'''Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36''', Kay B K, Peng H B. 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=204]
***detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
**'''The early development of Xenopus laevis. An atlas of the histology''', Hausen P, Riebesell M. 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=124]
***detailed histological sections of Xenopus embryonic development
**'''Atlas of Xenopus Development. ''', Bernardini, G, Prati, M, Bonetti, F, Scari, G, 1999 [http://www.xenbase.org/literature/book.do?method=display&bookId=202]
***scanning, transmission, and light microscopy images of Xenopus embryonic development
**'''Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology''', Seidman S, Soreq H. 1997 [http://www.xenbase.org/literature/book.do?method=display&bookId=203]
***referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
 
 
*'''Animal Husbandry/Animal Protocols'''
**[[inducing egg laying via hCG injection (Vize lab)]]
 
 
*'''In Situ Hybridization'''
**[[in situ hybridization, brief version (Harland lab)]]
**[[Fluorescent In-Situs and FCIS (Vize lab)]]
***[[Wholemount embryo FISH / Double FISH]]
***[[Flourescin Tyramide Synthesis]]
***[[Cy3 Tyramide Synthesis]]
***[[FCIS]]
***[[Chromosomal FISH-TSA]]
 
 
*'''Immunohisochemistry'''
**[[wholemount antibody staining (CSH Protocols) (Wallingford lab)]]
 
 
*'''Other Embryo Staining Protocols'''
**[[benzidine-peroxidase staining for blood (Thomsen lab)]]
**[[apoptosis via TUNEL (Veenstra lab)]]
 
 
*'''Other Immuno Protocols'''
**[[immunoprecipitation (Stukenberg lab)]]
**[[silver staining protein gels (Stukenberg lab)]]
**[[immunofluorescence with biotinylated antibodies (Stukenberg lab)]]
 
 
*'''[[Oocyte Transfer Technique (Heasman/Wylie labs)]]'''
**[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]]
**[[Part 2: ODN Modification]]
**[[Part 3: Host Transfer Technique]]
**[[Part 4: Fertilization and Development]]
 
 
*'''Xenopus Oocyte and Egg Extracts'''
**[[crude interphase egg extract (Shechter lab)]]
**[[isolation of chromatin from egg extract and histone recovery (Shechter lab)]]
**[[preparation of cycling egg extract (Shechter lab)]]
**[[cycling extracts, mike's protocol (Stukenberg lab)]]
**[[cycling extracts, sara's protocol (Stukenberg lab)]]
**[[CSF extract, mike's protocol (Stukenberg lab)]]
**[[CSF extract, sara's protocol (Stukenberg lab)]]
**[[assaying H1 kinase in extracts (Stukenberg lab)]]
 
 
*'''Xenopus Tissue Culture'''
**[[tissue culture of Xenopus cell lines S3, XTC (Stukenberg lab)]]
**[[isolation of mitotic chromosomes from XTC cells (Stukenberg lab)]]
**[[in-vitro kinetochore-microtubule binding assays (Stukenberg lab)]]
 
 
*'''Purifying Polyclonal Antibodies using Affinity Columns / Chromatography'''
**[[Making an Affinity Column]]
**[[Running an Affinity Column]]

Latest revision as of 06:00, 10 July 2010

glomerus

This is the community wiki page for the glomerus please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase