Protocols and XB-ANAT-145: Difference between pages

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*'''''Books for Xenopus Research and Protocols'''''
=glomerus=  
**'''Normal Table of Xenopus Laevis (Daudin)''', Nieuwkoop and Faber (Third edition), 1994 [http://www.xenbase.org/literature/book.do?method=display&bookId=1]
This is the community wiki page for the ''glomerus'' please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase
***a systematic and chronological description of Xenopus laevis development
**'''Early Development of Xenopus laevis: A Laboratory Manual''', Sive, Grainger, and Harland, 2000 [http://www.xenbase.org/literature/book.do?method=display&bookId=22]
***comprehensive collection of protocols for the study of early development in Xenopus embryos
**'''Xenopus Protocols: Cell Biology and Signal Transduction''', Liu (First edition), 2006 [http://www.xenbase.org/literature/book.do?method=display&bookId=206]
***step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
**'''Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36''', Kay and Peng, 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=204]
***detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
**'''Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology''', Seidman and Soreq, 1997 [http://www.xenbase.org/literature/book.do?method=display&bookId=203]
***referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
**'''Atlas of Xenopus Development''', Bernardini, Prati, Bonetti, and Scari, 1999 [http://www.xenbase.org/literature/book.do?method=display&bookId=202]
***scanning, transmission, and light microscopy images of Xenopus embryonic development
**'''The early development of Xenopus laevis. An atlas of the histology''', Hausen and Riebesell, 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=124]
***detailed histological sections of Xenopus embryonic development
**'''Color Atlas of Xenopus laevis Histology''', Wiechmann and Wirsig-Wiechmann, 2003 [http://www.xenbase.org/literature/book.do?method=display&bookId=82]
***central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis
 
 
*'''''[[Lab Solutions and Reagents]]'''''
 
 
*'''''Animal Husbandry/Animal Protocols'''''
**[[inducing egg laying via hCG injection (Vize lab)]]
**[[anesthesia, in vitro fertilization, and natural mating of Xenopus tropicalis (Conlon lab)]]
**[[in vitro fertilization of Xenopus laevis (Conlon lab)]]
**[[high-salt solution for egg laying (Zorn lab/Ron Laskey)]]
**[[dejellying embryos (Zorn lab)]]
 
 
*'''''Transgenics'''''
**[[transgenesis in Xenopus laevis - adapted from Amaya et al 2001 (Conlon lab)]]
**[[tadpole tail sampling for genomic DNA (Conlon lab)]]
 
 
*'''''In Situ Hybridization'''''
**[[in situ hybridization, brief version (Harland lab)]]
**[[whole-mount in situ hybridization (Conlon lab)]]
***[[in situ checklist (Conlon lab)]]
**[[fluorescent in-situs and FCIS (Vize lab)]]
***[[Wholemount embryo FISH / Double FISH]]
***[[Flourescin Tyramide Synthesis]]
***[[Cy3 Tyramide Synthesis]]
***[[FCIS]]
 
 
*'''''Immunohisochemistry'''''
**[[wholemount antibody staining (CSH Protocols) (Wallingford lab)]]
**[[wholemount antibody staining - alkaline phosphatase detection (Conlon lab)]]
**[[wholemount antibody staining - adapted from Dan Weeks' protocol (Conlon lab)]]
**[[immunostaining sectioned embryos (Conlon lab)]]
**[[hydrochloric acid antigen retrieval (Zorn lab)]]
 
 
*'''''Histology'''''
**[[cryosectioning Xenopus embryos (Conlon lab)]]
 
 
*'''''Other Embryo Staining Protocols'''''
**[[benzidine-peroxidase staining for blood (Conlon lab)]]
**[[benzidine-peroxidase staining for blood (Thomsen lab)]]
**[[apoptosis via TUNEL (Veenstra lab)]]
**[[TUNEL staining of whole Xenopus embryos - Harland protocol (Conlon lab)]]
 
 
*'''''Other Immuno Protocols and Protein Protocols'''''
**[[immunoprecipitation (Stukenberg lab)]]
**[[immunofluorescence with biotinylated antibodies (Stukenberg lab)]]
**Purifying Polyclonal Antibodies using Affinity Columns/Chromatography
***[[Making an Affinity Column]]
***[[Running an Affinity Column]]
**[[whole embryo protein extraction with freon (Conlon lab)]]
**[[SDS-PAGE gels (Conlon lab)]]
**[[western blot protocol (Conlon lab)]]
**[[silver staining protein gels (Stukenberg lab)]]
 
 
*'''''[[Oocyte Transfer Technique (Heasman/Wylie labs)]]'''''
**[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]]
**[[Part 2: ODN Modification]]
**[[Part 3: Host Transfer Technique]]
**[[Part 4: Fertilization and Development]]
 
 
*'''''Xenopus Oocyte and Egg Extracts'''''
**[[crude interphase egg extract (Shechter lab)]]
**[[isolation of chromatin from egg extract and histone recovery (Shechter lab)]]
**[[preparation of cycling egg extract (Shechter lab)]]
**[[cycling extracts, mike's protocol (Stukenberg lab)]]
**[[cycling extracts, sara's protocol (Stukenberg lab)]]
**[[CSF extract, mike's protocol (Stukenberg lab)]]
**[[CSF extract, sara's protocol (Stukenberg lab)]]
**[[assaying H1 kinase in extracts (Stukenberg lab)]]
 
 
*'''''Xenopus Tissue Culture'''''
**[[tissue culture of Xenopus cell lines S3, XTC (Stukenberg lab)]]
**[[isolation of mitotic chromosomes from XTC cells (Stukenberg lab)]]
**[[in-vitro kinetochore-microtubule binding assays (Stukenberg lab)]]

Latest revision as of 06:00, 10 July 2010

glomerus

This is the community wiki page for the glomerus please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase