Protocols

• Useful Books and Handbooks for Xenopus Research

• Animal Husbandry/Animal Protocols
  • inducing egg laying via hCG injection (Vize lab)

• In Situ Hybridization
  • in situ hybridization, brief version (Harland lab)
  • Fluorescent In-Situs and FCIS (Vize lab)
    • Wholemount embryo FISH / Double FISH
    • Flourescin Tyramide Synthesis
    • Cy3 Tyramide Synthesis
    • FCIS
    • Chromosomal FISH-TSA

• Immunohisochemistry
  • wholemount antibody staining (CSH Protocols) (Wallingford lab)

• Other Embryo Staining Protocols
  • benzidine-peroxidase staining for blood (Thomsen lab)
  • apoptosis via TUNEL (Veenstra lab)

• Other Immuno Protocols
  • immunoprecipitation (Stukenberg lab)
  • silver staining protein gels (Stukenberg lab)
  • immunofluorescence with biotinylated antibodies (Stukenberg lab)

• Oocyte Transfer Technique (Heasman/Wylie labs)
  • Part 1: Choosing Oligodeoxyribonucleotides (ODNs)
  • Part 2: ODN Modification
  • Part 3: Host Transfer Technique
  • Part 4: Fertilization and Development

• Xenopus Oocyte and Egg Extracts
  • crude interphase egg extract (Shechter lab)
  • isolation of chromatin from egg extract and histone recovery (Shechter lab)
  • preparation of cycling egg extract (Shechter lab)
  • cycling extracts, mike's protocol (Stukenberg lab)
  • cycling extracts, sara's protocol (Stukenberg lab)
  • CSF extract, mike's protocol (Stukenberg lab)
  • CSF extract, sara's protocol (Stukenberg lab)
  • assaying H1 kinase in extracts (Stukenberg lab)

• Xenopus Tissue Culture
  • tissue culture of Xenopus cell lines S3, XTC (Stukenberg lab)
  • isolation of mitotic chromosomes from XTC cells (Stukenberg lab)
  • in-vitro kinetochore-microtubule binding assays (Stukenberg lab)

• Purifying Polyclonal Antibodies using Affinity Columns / Chromatography
  • Making an Affinity Column
  • Running an Affinity Column