Protocols

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  • Online Xenopus Resources
    • Cold Spring Harbor Protocols - Xenopus [1], subscription required for 130+ articles, select articles shown below:
      • Housing and Feeding of Xenopus laevis - Sive et al. [2]
      • Inducing Ovulation in Xenopus laevis - Sive et al. [3]
      • Xenopus laevis In Vitro Fertilization and Natural Mating Methods - Sive et al. [4]
      • Egg Collection and In Vitro Fertilization of the Western Clawed Frog Xenopus tropicalis - Showell & Conlon [5]
      • Isolation of Xenopus Oocytes - Sive et al. [6]
      • Isolating Xenopus laevis Testes - Sive et al. [7]
      • Dejellying Xenopus laevis Embryos - Sive et al. [8]
      • Removing the Vitelline Membrane from Xenopus laevis Embryos - Sive et al. [9]
      • Microinjection of Xenopus Embryos - Sive et al. [10]
      • Defolliculation of Xenopus Oocytes - Sive et al. [11]
      • Microinjection of Xenopus Oocytes - Sive et al. [12]
      • Animal Cap Isolation from Xenopus laevis - Sive et al. [13]
      • Xenopus laevis Keller Explants - Sive et al. [14]
      • Microinjection of RNA and Preparation of Secreted Proteins from Xenopus Oocytes - Sive et al. [15]
      • Calibration of the Injection Volume for Microinjection of Xenopus Oocytes and Embryos - Sive et al. [16]
      • Isolation of DNA from Red Blood Cells in Xenopus - Sive et al. [17]
      • Investigating Morphogenesis in Xenopus Embryos: Imaging Strategies, Processing, and Analysis - Kim & Davidson [18]
      • Low-Magnification Live Imaging of Xenopus Embryos for Cell and Developmental Biology - Wallingford [19]
      • High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology - Keiserman et al. [20]
      • Preparation of Fixed Xenopus Embryos for Confocal Imaging - Wallingford [21]
      • Whole-Mount Fluorescence Immunocytochemistry on Xenopus Embryos - Lee et al. [22]
      • Generation of Transgenic Xenopus laevis - Kroll & Amaya - [23] [24] [25]
      • In Vivo Time-Lapse Imaging of Neuronal Development in Xenopus - Ruthazer et al. [26]
      • Photoconversion for Tracking the Dynamics of Cell Movement in Xenopus laevis Embryos - Chernet et al. [27]
      • Single-Cell Electroporation in Xenopus - Liu & Haas [28]
      • Imaging Axon Pathfinding in Xenopus In Vivo - Leung & Holt [29]
      • A Versatile Protocol for mRNA Electroporation of Xenopus laevis Embryos - Chernet & Levin [30]


    • The Journal of Visualized Experiments (JOVE) - Xenopus embryo [31] - excellent video demonstrations
      • Obtaining Eggs from Xenopus laevis Females - Cross & Powers [32]
      • Microinjection of Xenopus Laevis Oocytes - Cohen et al. - [33]
      • Fertilization of Xenopus oocytes, Host Transfer Method - Schneider et al. [34]
      • Tissue Determination Using Animal Cap Transplant (ACT) Assay in X. laevis - Viczian & Zuber [35]
      • Blastomere Explants to Test for Cell Fate Commitment - Grant et al. [36]
      • Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation - Amaya & Kroll [37]
      • Organizer and Animal Pole Explants from X. laevis Embryos and Cell Adhesion Assay - Ogata & Cho [38]
      • Plastic Embedding and Sectioning of X. laevis Embryos - Ogata1 et al. [39]
      • Visualizing RNA Localization in Xenopus Oocytes - Gagnon & Mowry [40]
      • Neural Explant Cultures from Xenopus laevis - Lowery et al. [41]
      • Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling - Itoh & Sokol [42]
      • Dissection, Culture, and Analysis of X. laevis Embryonic Retinal Tissue - McDonough et al. [43]
      • X. tropicalis Egg Extracts to Identify Microtubule-associated RNAs - Sharp & Blower [44]
      • Electroporation of Craniofacial Mesenchyme - Tabler & Liu [45]
      • Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures - Yazejian et al. [46]
      • Single Cell Electroporation in vivo within the Intact Developing Brain - Hewapathirane & Haas - [47]
      • Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells - Joshi & Davidson [48]
      • Preparation and Fractionation of Xenopus laevis Egg Extracts - Cross & Powers [49]
      • In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts - Cross & Powers [50]
      • Study of the DNA Damage Checkpoint using Xenopus Egg Extracts - Willis et al. [51]
      • Two Types of Assays for Detecting Frog Sperm Chemoattraction - Burnett et al. [52]
      • Comparative in vivo Study of gp96 Adjuvanticity in X. laevis - Nedelkovska et al. [53]
      • Patch Clamp and Perfusion Techniques - Yang et al. [54]
      • Patch Clamp Recording of Ion Channels - Brown et al. [55]
      • Cation Transport in Xenopus Oocytes - Dürr et al.[56]


  • Books for Xenopus Research and Protocols
    • Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [57]
      • a systematic and chronological description of Xenopus laevis development
    • Early Development of Xenopus laevis: A Laboratory Manual, Sive, Grainger, and Harland, 2000 [58]
      • comprehensive collection of protocols for the study of early development in Xenopus embryos
    • The Laboratory Xenopus sp., Green, 2010 [59]
      • a highly detailed manual containing Xenopus husbandry, management, veterinary care, and frog and equipment vendor information
    • Xenopus Protocols: Cell Biology and Signal Transduction, Liu (First edition), 2006 [60]
      • step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
    • Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36, Kay and Peng, 1991 [61]
      • detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
    • Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology, Seidman and Soreq, 1997 [62]
      • referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
    • Atlas of Xenopus Development, Bernardini, Prati, Bonetti, and Scari, 1999 [63]
      • scanning, transmission, and light microscopy images of Xenopus embryonic development
    • The early development of Xenopus laevis. An atlas of the histology, Hausen and Riebesell, 1991 [64]
      • detailed histological sections of Xenopus embryonic development
    • Color Atlas of Xenopus laevis Histology, Wiechmann and Wirsig-Wiechmann, 2003 [65]
      • central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis