Xenopus development time / temperature charts and Alpha-tubulin - microtubules: Difference between pages

From XenWiki
(Difference between pages)
Jump to navigation Jump to search
No edit summary
 
No edit summary
 
Line 1: Line 1:
Xenbase would like to thank Mustafa Khokha for making his data for these charts available to us.  <br/><br/>Original reference:<br/>
'''Antigen:''' microtubules from chick embryo brain
'''Epitope:'''
'''Host Species/Isotype:''' mouse IgG
'''Antigen Species:''' chicken
'''Applications:'''
'''Antibody Name:''' DM1A
'''Supplier:''' Sigma (cat#T9026)
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
'''Contributor:''' Wallingford Lab


Khokha, M. K., Chung, C., Bustamante, E. L., Gaw, L. W., Trott, K. A., Yeh, J., Lim, N., Lin, J. C., Taverner, N., Amaya, E., Papalopulu, N.,  
applications abbreviations:
Smith, J. C., Zorn, A. M., Harland, R. M. and Grammer, T. C. (2002), Techniques and probes for the study of Xenopus tropicalis development. Developmental Dynamics, 225: 499–510. doi: 10.1002/dvdy.10184 [http://www.ncbi.nlm.nih.gov/pubmed/12454926?dopt=Abstract]
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry


Additional Comments:
'''Available as:''' Ascites


<h2><i>Xenopus tropicalis</i></h2>
'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.


{| border="1" class="tableizer-table"  class="tableizer-firstrow"
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
!
!  colspan=3 align="center" | 16°C
!  colspan=3 align="center" | 18°C
!  colspan=3 align="center" | 22°C
!  colspan=3 align="center" | 25°C
!  colspan=3 align="center" | 28°C
!  colspan=3 align="center" | 30°C
|-
| <b>NF Stage</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
|-
| 1
| 1 hr 3 min
| 30 min
| 1 hr 39 min
| 1 hr 26 min
| 1 hr 4 min
| 1 hr 42 min
| 0 hr 31 min
| 0 hr 31 min
| 0 hr 31 min
| 0 hr 60 min
| 0 hr 31 min
| 1 hr 18 min
| 1 hr 1 min
| 1 hr 1 min
| 1 hr 1 min
| 0 hr 39 min
| 0 hr 31 min
| 0 hr 52 min
|-
| 2
| 2 hr 5 min
| 1 hr 24 min
| 3 hr 39 min
| 2 hr 15 min
| 1 hr 26 min
| 3 hr 34 min
| 1 hr 33 min
| 1 hr 19 min
| 1 hr 55 min
| 1 hr 32 min
| 1 hr 13 min
| 1 hr 59 min
| 1 hr 23 min
| 1 hr 1 min
| 1 hr 56 min
| 1 hr 22 min
| 1 hr 5 min
| 1 hr 46 min
|-
| 3
| 3 hr 33 min
| 2 hr 18 min
| 6 hr 0 min
| 3 hr 13 min
| 2 hr 31 min
| 4 hr 27 min
| 1 hr 54 min
| 1 hr 34 min
| 2 hr 20 min
| 1 hr 51 min
| 1 hr 21 min
| 2 hr 17 min
| 1 hr 39 min
| 1 hr 17 min
| 2 hr 15 min
| 1 hr 45 min
| 1 hr 22 min
| 2 hr 1 min
|-
| 4
| 3 hr 33 min
| 2 hr 42 min
| 6 hr 0 min
| 4 hr 39 min
| 3 hr 50 min
| 6 hr 57 min
| 2 hr 24 min
| 2 hr 11 min
| 2 hr 50 min
| 2 hr 17 min
| 1 hr 34 min
| 2 hr 42 min
| 1 hr 58 min
| 1 hr 38 min
| 2 hr 28 min
| 1 hr 47 min
| 1 hr 22 min
| 2 hr 12 min
|-
| 5
| 3 hr 53 min
| 3 hr 23 min
| 4 hr 35 min
| N/A
| N/A
| N/A
| 2 hr 44 min
| 2 hr 32 min
| 2 hr 55 min
| 2 hr 44 min
| 2 hr 20 min
| 3 hr 2 min
| 2 hr 17 min
| 1 hr 47 min
| 2 hr 42 min
| 2 hr 7 min
| 1 hr 40 min
| 2 hr 41 min
|-
| 6
| 12 hr 5 min
| 3 hr 39 min
| 26 hr 4 min
| 7 hr 30 min
| 6 hr 5 min
| 8 hr 22 min
| 3 hr 60 min
| 2 hr 51 min
| 4 hr 20 min
| 3 hr 18 min
| 2 hr 39 min
| 4 hr 10 min
| 2 hr 48 min
| 2 hr 28 min
| 3 hr 20 min
| 2 hr 43 min
| 2 hr 21 min
| 3 hr 7 min
|-
| 6.5
| 15 hr 22 min
| 4 hr 17 min
| 26 hr 4 min
| 15 hr 31 min
| 6 hr 5 min
| 24 hr 3 min
| 3 hr 44 min
| 2 hr 51 min
| 4 hr 30 min
| 3 hr 41 min
| 2 hr 53 min
| 4 hr 49 min
| 3 hr 9 min
| 2 hr 38 min
| 3 hr 50 min
| 3 hr 2 min
| 2 hr 21 min
| 3 hr 50 min
|-
| 7
| 16 hr 28 min
| 4 hr 51 min
| 26 hr 4 min
| 20 hr 7 min
| 11 hr 39 min
| 24 hr 3 min
| 4 hr 1 min
| 3 hr 26 min
| 4 hr 30 min
| 4 hr 3 min
| 2 hr 58 min
| 4 hr 49 min
| 3 hr 28 min
| 3 hr 7 min
| 3 hr 50 min
| 3 hr 19 min
| 2 hr 21 min
| 3 hr 50 min
|-
| 8
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 5 hr 7 min
| 4 hr 24 min
| 6 hr 16 min
| 5 hr 14 min
| 4 hr 0 min
| 6 hr 5 min
| 4 hr 39 min
| 4 hr 8 min
| 5 hr 32 min
| 4 hr 2 min
| 3 hr 24 min
| 4 hr 45 min
|-
| 9
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 7 hr 54 min
| 5 hr 29 min
| 12 hr 6 min
| 6 hr 55 min
| 4 hr 58 min
| 9 hr 22 min
| 6 hr 17 min
| 4 hr 49 min
| 7 hr 25 min
| 5 hr 49 min
| 3 hr 51 min
| 7 hr 27 min
|-
| 10
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 13 hr 17 min
| 9 hr 49 min
| 17 hr 3 min
| 9 hr 25 min
| 6 hr 57 min
| 11 hr 13 min
| 7 hr 48 min
| 6 hr 34 min
| 8 hr 22 min
| 7 hr 46 min
| 7 hr 27 min
| 8 hr 17 min
|-
| 10.5
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 15 hr 12 min
| 9 hr 49 min
| 18 hr 4 min
| 10 hr 27 min
| 8 hr 22 min
| 11 hr 13 min
| 8 hr 1 min
| 7 hr 25 min
| 8 hr 22 min
| 8 hr 32 min
| 8 hr 17 min
| 9 hr 2 min
|-
| 11
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 15 hr 54 min
| 13 hr 37 min
| 19 hr 14 min
| 11 hr 16 min
| 10 hr 24 min
| 12 hr 11 min
| 8 hr 8 min
| 7 hr 48 min
| 8 hr 22 min
| 8 hr 43 min
| 8 hr 17 min
| 9 hr 2 min
|-
| 11.5
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 16 hr 42 min
| 13 hr 37 min
| 19 hr 14 min
| 11 hr 9 min
| 9 hr 4 min
| 12 hr 11 min
| 9 hr 19 min
| 9 hr 19 min
| 9 hr 19 min
| 9 hr 22 min
| 8 hr 17 min
| 10 hr 7 min
|-
| 12
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 18 hr 8 min
| 15 hr 52 min
| 20 hr 4 min
| 11 hr 24 min
| 9 hr 4 min
| 14 hr 10 min
| 9 hr 43 min
| 9 hr 19 min
| 10 hr 30 min
| 10 hr 7 min
| 10 hr 7 min
| 10 hr 7 min
|-
| 12.5
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 18 hr 38 min
| 16 hr 45 min
| 20 hr 4 min
| 12 hr 7 min
| 9 hr 4 min
| 14 hr 10 min
| 10 hr 21 min
| 9 hr 19 min
| 11 hr 5 min
| N/A
| N/A
| N/A
|-
| 13
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 19 hr 6 min
| 17 hr 26 min
| 20 hr 28 min
| 12 hr 14 min
| 10 hr 46 min
| 14 hr 10 min
| 10 hr 53 min
| 10 hr 30 min
| 11 hr 5 min
| N/A
| N/A
| N/A
|-
| 14
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 19 hr 24 min
| 18 hr 16 min
| 20 hr 28 min
| 15 hr 14 min
| 15 hr 14 min
| 15 hr 14 min
| N/A
| N/A
| N/A
| N/A
| N/A
| N/A
|-
| 15
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 20 hr 19 min
| 19 hr 3 min
| 22 hr 22 min
| 13 hr 25 min
| 12 hr 25 min
| 15 hr 14 min
| N/A
| N/A
| N/A
| N/A
| N/A
| N/A
|-
| 16
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 21 hr 56 min
| 20 hr 17 min
| 23 hr 10 min
| 14 hr 2 min
| 13 hr 12 min
| 16 hr 10 min
| N/A
| N/A
| N/A
| N/A
| N/A
| N/A
|-
| 17
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 23 hr 9 min
| 21 hr 1 min
| 24 hr 32 min
| 14 hr 52 min
| 12 hr 59 min
| 17 hr 17 min
| 12 hr 25 min
| 12 hr 18 min
| 12 hr 47 min
| 10 hr 58 min
| 10 hr 58 min
| 10 hr 58 min
|-
| 18
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 22 hr 57 min
| 21 hr 1 min
| 27 hr 40 min
| 15 hr 34 min
| 14 hr 16 min
| 17 hr 9 min
| 12 hr 40 min
| 11 hr 29 min
| 13 hr 45 min
| 11 hr 24 min
| 11 hr 24 min
| 11 hr 24 min
|-
| 19
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 25 hr 25 min
| 22 hr 11 min
| 28 hr 14 min
| 17 hr 44 min
| 15 hr 27 min
| 21 hr 28 min
| 12 hr 38 min
| 12 hr 38 min
| 12 hr 38 min
| N/A
| N/A
| N/A
|-
| 20
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 26 hr 25 min
| 23 hr 15 min
| 28 hr 14 min
| 18 hr 27 min
| 13 hr 40 min
| 22 hr 39 min
| 13 hr 14 min
| 13 hr 14 min
| 13 hr 14 min
| 12 hr 22 min
| 12 hr 22 min
| 12 hr 22 min
|-
| 21
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 23 hr 32 min
| 23 hr 15 min
| 23 hr 39 min
| 20 hr 35 min
| 16 hr 31 min
| 26 hr 10 min
| 15 hr 10 min
| 14 hr 25 min
| 16 hr 18 min
| 13 hr 7 min
| 12 hr 46 min
| 13 hr 49 min
|-
| 22
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 29 hr 23 min
| 25 hr 17 min
| 30 hr 59 min
| 21 hr 28 min
| 17 hr 47 min
| 28 hr 4 min
| N/A
| N/A
| N/A
| 13 hr 60 min
| 13 hr 18 min
| 15 hr 4 min
|-
| 23
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 22 hr 8 min
| 17 hr 22 min
| 28 hr 4 min
| 15 hr 29 min
| 14 hr 58 min
| 17 hr 3 min
| 14 hr 19 min
| 13 hr 57 min
| 15 hr 4 min
|-
| 24
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 22 hr 13 min
| 18 hr 8 min
| 27 hr 13 min
| 17 hr 11 min
| 15 hr 51 min
| 18 hr 54 min
| 15 hr 56 min
| 14 hr 58 min
| 16 hr 50 min
|-
| 25
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 22 hr 12 min
| 18 hr 56 min
| 29 hr 4 min
| 18 hr 52 min
| 17 hr 42 min
| 19 hr 58 min
| 17 hr 2 min
| 16 hr 25 min
| 17 hr 42 min
|-
| 26
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 27 hr 0 min
| 23 hr 16 min
| 30 hr 0 min
| 74 hr 15 min
| 20 hr 37 min
| 22 hr 36 min
| 17 hr 43 min
| 16 hr 59 min
| 18 hr 22 min
|-
| 27
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 31 hr 2 min
| 30 hr 59 min
| 31 hr 4 min
| 20 hr 26 min
| 18 hr 33 min
| 22 hr 36 min
| 18 hr 9 min
| 18 hr 0 min
| 18 hr 25 min
|-
| 28
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 25 hr 1 min
| 25 hr 1 min
| 25 hr 1 min
| 23 hr 45 min
| 23 hr 18 min
| 24 hr 3 min
| 19 hr 40 min
| 18 hr 47 min
| 21 hr 12 min
|-
| 29
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 19 hr 49 min
| 19 hr 18 min
| 20 hr 20 min
|-
| 30
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 20 hr 31 min
| 19 hr 18 min
| 21 hr 53 min
|-
| 31
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 20 hr 28 min
| 20 hr 21 min
| 20 hr 54 min
|-
| 32
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 21 hr 35 min
| 20 hr 11 min
| 23 hr 7 min
|-
| 33
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 23 hr 55 min
| 21 hr 1 min
| 27 hr 0 min
|-
| 34
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 25 hr 42 min
| 22 hr 43 min
| 27 hr 34 min
|-
| 35
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 28 hr 31 min
| 24 hr 53 min
| 30 hr 40 min
|-
| 36
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| N/A
| N/A
| N/A
|-
| 37
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 30 hr 55 min
| 30 hr 55 min
| 30 hr 55 min
|-
| 37
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| N/A
| N/A
| N/A
|-
| 37
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 30 hr 55 min
| 30 hr 55 min
| 30 hr 55 min
|}<h2><i>Xenopus laevis</i></h2>
{|  class="tableizer-table"  class="tableizer-firstrow"
!
!  colspan=3 align="center" | 16°C
!  colspan=3 align="center" | 18°C
!  colspan=3 align="center" | 22°C
!  colspan=3 align="center" | 25°C
!  colspan=3 align="center" | 28°C
|-
| <b>NF Stage</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
| <b>Average</b>
| <b>Minimum</b>
| <b>Maximum</b>
|-
| 1
| 0 hr 53 min
| 0 hr 43 min
| 1 hr 9 min
| 0 hr 44 min
| 0 hr 42 min
| 0 hr 47 min
| 0 hr 45 min
| 0 hr 45 min
| 0 hr 45 min
| 1 hr 16 min
| 1 hr 16 min
| 1 hr 16 min
| 0 hr 53 min
| 0 hr 47 min
| 1 hr 0 min
|-
| 2
| 2 hr 8 min
| 1 hr 50 min
| 2 hr 43 min
| 1 hr 59 min
| 1 hr 42 min
| 2 hr 37 min
| 1 hr 33 min
| 1 hr 32 min
| 2 hr 48 min
| 1 hr 34 min
| 1 hr 30 min
| 1 hr 38 min
| 1 hr 47 min
| 1 hr 47 min
| 1 hr 47 min
|-
| 3
| 2 hr 42 min
| 2 hr 40 min
| 2 hr 43 min
| 2 hr 38 min
| 2 hr 37 min
| 2 hr 39 min
| 2 hr 6 min
| 2 hr 6 min
| 2 hr 6 min
| 2 hr 13 min
| 2 hr 13 min
| 2 hr 13 min
| 1 hr 42 min
| 1 hr 39 min
| 1 hr 47 min
|-
| 4
| 3 hr 52 min
| 3 hr 50 min
| 3 hr 54 min
| 3 hr 49 min
| 3 hr 45 min
| 3 hr 53 min
| 2 hr 41 min
| 2 hr 34 min
| 2 hr 48 min
| 2 hr 20 min
| 2 hr 13 min
| 2 hr 25 min
| 2 hr 18 min
| 2 hr 1 min
| 2 hr 30 min
|-
| 5
| 4 hr 43 min
| 4 hr 31 min
| 4 hr 54 min
| 4 hr 39 min
| 4 hr 29 min
| 4 hr 49 min
| N/A
| N/A
| N/A
| 2 hr 50 min
| 2 hr 50 min
| 2 hr 50 min
| 2 hr 45 min
| 2 hr 30 min
| 2 hr 50 min
|-
| 6
| 6 hr 21 min
| 5 hr 49 min
| 6 hr 52 min
| 6 hr 16 min
| 5 hr 45 min
| 5 hr 45 min
| 3 hr 58 min
| 3 hr 40 min
| 4 hr 25 min
| 3 hr 52 min
| 3 hr 30 min
| 4 hr 23 min
| 3 hr 27 min
| 3 hr 22 min
| 3 hr 32 min
|-
| 6.5
| 6 hr 40 min
| 5 hr 49 min
| 9 hr 10 min
| 7 hr 56 min
| 5 hr 45 min
| 9 hr 33 min
| 4 hr 10 min
| 3 hr 40 min
| 4 hr 43 min
| 4 hr 19 min
| 3 hr 30 min
| 5 hr 22 min
| 3 hr 55 min
| 3 hr 22 min
| 4 hr 31 min
|-
| 7
| 9 hr 5 min
| 7 hr 47 min
| 10 hr 34 min
| 8 hr 46 min
| 7 hr 44 min
| 9 hr 33 min
| 5 hr 2 min
| 4 hr 43 min
| 5 hr 41 min
| 4 hr 47 min
| 4 hr 23 min
| 5 hr 22 min
| 4 hr 22 min
| 4 hr 13 min
| 4 hr 31 min
|-
| 8
| 12 hr 4 min
| 9 hr 33 min
| 13 hr 35 min
| 11 hr 21 min
| 10 hr 22 min
| 12 hr 22 min
| 6 hr 20 min
| 5 hr 41 min
| 6 hr 39 min
| N/A
| N/A
| N/A
| 5 hr 13 min
| 5 hr 13 min
| 5 hr 13 min
|-
| 9
| 16 hr 42 min
| 13 hr 48 min
| 19 hr 23 min
| 13 hr 57 min
| 10 hr 36 min
| 16 hr 25 min
| 8 hr 40 min
| 7 hr 37 min
| 9 hr 28 min
| 7 hr 25 min
| 6 hr 34 min
| 8 hr 32 min
| 6 hr 34 min
| 6 hr 6 min
| 7 hr 1 min
|-
| 10
| 20 hr 22 min
| 20 hr 19 min
| 24 hr 0 min
| 17 hr 52 min
| 16 hr 57 min
| 19 hr 16 min
| 10 hr 37 min
| 10 hr 21 min
| 11 hr 10 min
| 9 hr 33 min
| 8 hr 43 min
| 10 hr 33 min
| 9 hr 3 min
| 8 hr 21 min
| 10 hr 27 min
|-
| 10.5
| 21 hr 23 min
| 19 hr 56 min
| 24 hr 0 min
| 18 hr 45 min
| 16 hr 57 min
| 20 hr 49 min
| 11 hr 29 min
| 10 hr 21 min
| 12 hr 41 min
| 9 hr 42 min
| 8 hr 43 min
| 10 hr 33 min
| 9 hr 30 min
| 8 hr 21 min
| 10 hr 27 min
|-
| 11
| 22 hr 13 min
| 19 hr 56 min
| 24 hr 0 min
| 19 hr 53 min
| 16 hr 57 min
| 22 hr 24 min
| 12 hr 38 min
| 11 hr 10 min
| 14 hr 21 min
| 10 hr 15 min
| 10 hr 9 min
| 10 hr 28 min
| 9 hr 23 min
| 8 hr 55 min
| 10 hr 27 min
|-
| 11.5
| 23 hr 8 min
| 21 hr 55 min
| 24 hr 0 min
| 21 hr 18 min
| 17 hr 56 min
| 24 hr 0 min
| 13 hr 53 min
| 12 hr 2 min
| 17 hr 14 min
| 10 hr 28 min
| 10 hr 28 min
| 10 hr 28 min
| 9 hr 27 min
| 8 hr 55 min
| 10 hr 5 min
|-
| 12
| &nbsp;
| &nbsp;
| &nbsp;
| 22 hr 36 min
| 20 hr 49 min
| 24 hr 0 min
| 14 hr 57 min
| 12 hr 15 min
| 18 hr 14 min
| 12 hr 41 min
| 12 hr 1 min
| 13 hr 47 min
| 10 hr 5 min
| 10 hr 5 min
| 10 hr 5 min
|-
| 12.5
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 16 hr 21 min
| 13 hr 12 min
| 19 hr 12 min
| 12 hr 56 min
| 11 hr 35 min
| 14 hr 16 min
| N/A
| N/A
| N/A
|-
| 13
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 19 hr 4 min
| 18 hr 57 min
| 19 hr 12 min
| 13 hr 1 min
| 11 hr 35 min
| 14 hr 16 min
| N/A
| N/A
| N/A
|-
| 14
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 20 hr 8 min
| 17 hr 51 min
| 22 hr 7 min
| 14 hr 33 min
| 12 hr 1 min
| 16 hr 9 min
| N/A
| N/A
| N/A
|-
| 15
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 20 hr 55 min
| 20 hr 47 min
| 21 hr 3 min
| 16 hr 53 min
| 16 hr 50 min
| 16 hr 57 min
| N/A
| N/A
| N/A
|-
| 16
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 20 hr 44 min
| 20 hr 44 min
| 20 hr 44 min
| N/A
| N/A
| N/A
| N/A
| N/A
| N/A
|-
| 17
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 18 hr 42 min
| 18 hr 42 min
| 18 hr 42 min
| 17 hr 39 min
| 17 hr 39 min
| 17 hr 39 min
| 13 hr 44 min
| 13 hr 23 min
| 14 hr 4 min
|-
| 18
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 22 hr 11 min
| 19 hr 48 min
| 24 hr 0 min
| 18 hr 20 min
| 18 hr 9 min
| 19 hr 42 min
| 14 hr 38 min
| 14 hr 0 min
| 15 hr 17 min
|-
| 19
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 23 hr 57 min
| 23 hr 57 min
| 23 hr 57 min
| 18 hr 36 min
| 17 hr 1 min
| 19 hr 42 min
| 15 hr 8 min
| 13 hr 34 min
| 16 hr 54 min
|-
| 20
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| N/A
| N/A
| N/A
| 20 hr 32 min
| 18 hr 57 min
| 22 hr 5 min
| 16 hr 16 min
| 15 hr 32 min
| 16 hr 50 min
|-
| 21
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| N/A
| N/A
| N/A
| 22 hr 60 min
| 21 hr 32 min
| 24 hr 0 min
| 17 hr 37 min
| 17 hr 37 min
| 17 hr 37 min
|-
| 22
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 21 hr 47 min
| 21 hr 47 min
| 21 hr 47 min
| 20 hr 16 min
| 20 hr 16 min
| 20 hr 16 min
| 18 hr 50 min
| 18 hr 50 min
| 18 hr 50 min
|-
| 23
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 20 hr 44 min
| 20 hr 44 min
| 20 hr 44 min
| 18 hr 46 min
| 17 hr 36 min
| 20 hr 11 min
|-
| 24
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 22 hr 23 min
| 22 hr 23 min
| 22 hr 23 min
| 20 hr 42 min
| 20 hr 42 min
| 20 hr 42 min
|-
| 25
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 21 hr 39 min
| 19 hr 56 min
| 23 hr 48 min
|-
| 26
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| N/A
| N/A
| N/A
|-
| 27
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| &nbsp;
| 21 hr 56 min
| 21 hr 29 min
| 22 hr 23 min
|}

Latest revision as of 14:01, 4 February 2011

Antigen: microtubules from chick embryo brain

Epitope:

Host Species/Isotype: mouse IgG

Antigen Species: chicken

Applications:

Antibody Name: DM1A

Supplier: Sigma (cat#T9026)

Comments: fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ

Contributor: Wallingford Lab


applications abbreviations: WB - Western blotting, IP - immunoprecipitation, IF - immunofluorescence, IHC - immunohistochemistry, EMSA - electromobility shift assay, ChIP - chromatin immunoprecipitation, FCM - flow cytometry

Additional Comments:

Available as: Ascites

Optimized conditions: We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.

Other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse