Alpha-tubulin - microtubules and ECL - pronephric nephrons: Difference between pages

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(Created page with "'''Erythrina Cristagalli Lectin (ECL)''' Vector labs FL-1141 (fluorescein conjugated). [[Image:ECL_1.jpg|thumb|alt=ALT TEXT|Xenopus laevis embryo stained with fluorescein conj...")
 
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'''Antigen:''' microtubules from chick embryo brain
'''Erythrina Cristagalli Lectin (ECL)'''
'''Epitope:'''
'''Host Species/Isotype:''' mouse IgG
'''Antigen Species:''' chicken
'''Applications:'''
'''Antibody Name:''' DM1A
'''Supplier:''' Sigma (cat#T9026)
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
'''Contributor:''' Wallingford Lab


applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry


Additional Comments:
Vector labs FL-1141 (fluorescein conjugated).
[[Image:ECL_1.jpg|thumb|alt=ALT TEXT|Xenopus laevis embryo stained with fluorescein conjugated ECL (Image from P. Vize [http://www.xenbase.org/community/person.do?method=display&personId=702&tabId=0])]]
'''Available as:''' Ascites


'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.
Stains the proximal pronephric tubules and cement gland. Somite borders also weakly stained. Can be used as a counterstain on embryos already processed via in situ hybridization.
 
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse

Latest revision as of 10:50, 4 February 2011

Erythrina Cristagalli Lectin (ECL)


Vector labs FL-1141 (fluorescein conjugated).

ALT TEXT
Xenopus laevis embryo stained with fluorescein conjugated ECL (Image from P. Vize [1])

Stains the proximal pronephric tubules and cement gland. Somite borders also weakly stained. Can be used as a counterstain on embryos already processed via in situ hybridization.

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