Troponin - heart and other muscle and 4A6 – pronephros: Difference between pages

From XenWiki
(Difference between pages)
Jump to navigation Jump to search
No edit summary
 
No edit summary
 
Line 1: Line 1:
'''Protein/Antigen: tropomyosin, muscle '''
'''Antigen:''' dissected pronephroi, target unknown
'''Epitope: '''
'''Xenbase Gene Page:'''
'''Applications: IF '''
'''Epitope:''' mouse monoclonal IgG
'''Species raised against: chicken '''
'''Host Species/Isotype:'''
'''Isotype: mouse monoclonal IgG1 '''
'''Antigen Species:'''
'''Antibody Name: CH1 '''
'''Applications:''' IF, IP, WB
'''Supplier: DSHB '''
'''Antibody Name/Catalog Number:''' 4A6
'''Comments: works well in IF, fixative Dent's or PFA '''
'''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
'''Contributor: Sandra Koller and Dan Weeks '''
'''Comments:''' not sentitive to bleaching, does not function post in situ
'''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
'''Additional Information:'''
'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.
''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.
'''Fixatives:''' MEMFA
''Available as:''' cell supernatant, 3 x
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense.  As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.
'''Other species:''' Xenopus only
'''Reference(s):''' Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Revision as of 13:05, 8 March 2011

Antigen: dissected pronephroi, target unknown

Xenbase Gene Page:

Epitope: mouse monoclonal IgG

Host Species/Isotype:

Antigen Species:

Applications: IF, IP, WB

Antibody Name/Catalog Number: 4A6

Supplier: Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.

Comments: not sentitive to bleaching, does not function post in situ

Contributor: Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]


Additional Information: Developmental Window: First detects target at stage 39, continues until latest stages tested. Stains:' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias. Fixatives: MEMFA Available as:' cell supernatant, 3 x Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. Other species: Xenopus only

Reference(s): Dev Bio 171:531- [3]


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

Retrieved from "https://wiki.xenbase.org/xenwiki/index.php?title=Troponin_-_heart_and_other_muscle&oldid=47060"