4A6 – pronephros and Alpha-tubulin - tuba4b - microtubules: Difference between pages

Revision as of 14:13, 4 February 2011

Antigen: microtubules from chick embryo brain

Epitope:

Host Species/Isotype: mouse IgG

Antigen Species: chicken

Applications:

Antibody Name: DM1A

Supplier: Sigma (cat#T9026)

Comments: fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ

Contributor: Wallingford Lab

applications abbreviations: WB - Western blotting, IP - immunoprecipitation, IF - immunofluorescence, IHC - immunohistochemistry, EMSA - electromobility shift assay, ChIP - chromatin immunoprecipitation, FCM - flow cytometry

Additional Comments:

Available as: Ascites

Optimized conditions: We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.

Other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse

@@ Line 1: / Line 1: @@
-'''Antigen:'''		dissected pronephroi, target unknown
+'''Antigen:'''			microtubules from chick embryo brain
-'''Xenbase Gene Page:'''
+'''Epitope:'''
-'''Epitope:'''		mouse monoclonal IgG
+'''Host Species/Isotype:'''			mouse IgG
-'''Host Species/Isotype:'''
+'''Antigen Species:'''			chicken
-'''Antigen Species:'''
+'''Applications:'''
-'''Applications:'''		IF, IP, WB
+'''Antibody Name:'''			DM1A
-'''Antibody Name/Catalog Number:'''		4A6
+'''Supplier:'''			Sigma (cat#T9026)
-'''Supplier:'''		Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
+'''Comments:'''			fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
-'''Comments:'''		not sentitive to bleaching, does not function post in situ
+'''Contributor:'''			Wallingford Lab
-'''Contributor:'''		Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
+applications abbreviations:
-[[File:4A6.jpg]]
+WB - Western blotting,
+IP - immunoprecipitation,
-'''Additional Information:'''
+IF - immunofluorescence,
-		'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.
+IHC - immunohistochemistry,
-		''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.
+EMSA - electromobility shift assay,
-		'''Fixatives:''' MEMFA
+ChIP - chromatin immunoprecipitation,
-		''Available as:''' cell supernatant, 3 x
+FCM - flow cytometry
-		'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense.  As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.
-		'''Other species:''' Xenopus only
+Additional Comments:
-'''Reference(s):'''		Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
+'''Available as:''' Ascites
+'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.
-applications abbreviations:
+'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
-WB - Western blotting,
-IP - immunoprecipitation,
-IF - immunofluorescence,
-IHC - immunohistochemistry,
-EMSA - electromobility shift assay,
-ChIP - chromatin immunoprecipitation,
-FCM - flow cytometry

4A6 – pronephros and Alpha-tubulin - tuba4b - microtubules: Difference between pages

Revision as of 14:13, 4 February 2011

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