LMO2 - lmo2 - blood, presomitic mesoderm, CNS and C-myc - myc - from roche: Difference between pages

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'''Antigen:''' human lim domain only 2
'''Antigen:''' c-myc
 
'''Epitope:''' N-terminus aa 1-100, synthetic peptide
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6052813 myc]
'''Host Species/Isotype:''' rabbit polyclonal
'''Epitope:''' aa 408-439 of human c-myc
'''Host Species/Isotype:''' mouse monoclonal
'''Antigen Species:''' human
'''Antigen Species:''' human
'''Applications:''' WB
'''Applications:''' IF
 
'''Antibody Name:''' ab72841 [http://www.abcam.com/LMO2-antibody-ChIP-Grade-ab72841.html]
'''Antibody Name/Catalog Number:''' [https://www.roche-applied-science.com/servlet/RCProductDisplay?storeId=10202&catalogId=10202&langId=-1&countryId=us&forCountryId=us&productId=3.7.2.12.10.1 anti-c-myc (clone 9E10) 1 667 203]
 
'''Supplier:''' abcam
'''Supplier:''' Roche
 
'''Comments:''' works on WB, but not well
'''Comments:''' IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, 0.5 ug/ml use at 1:500 to 1:1000
 
'''Contributor:''' Aldo Ciau-Uitz
'''Contributor:''' Zorn Lab
 
'''Additional Information:''' reacts with several proteins, non of the predicted size for Xenopus Lmo2, Lmo2 MO has no effect on any of them
'''Additional Information:'''
'''Reference(s):'''
'''Reference(s):'''
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IP - immunoprecipitation,
IP - immunoprecipitation,
 
IF - immunofluorescence,
IF - immunofluorescence,
 
IHC - immunohistochemistry,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry
FCM - flow cytometry

Latest revision as of 12:05, 27 April 2011

Antigen: c-myc

Xenbase Gene Page: myc

Epitope: aa 408-439 of human c-myc

Host Species/Isotype: mouse monoclonal

Antigen Species: human

Applications: IF

Antibody Name/Catalog Number: anti-c-myc (clone 9E10) 1 667 203

Supplier: Roche

Comments: IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, 0.5 ug/ml use at 1:500 to 1:1000

Contributor: Zorn Lab

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

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