C-myc - myc - from roche and Anti-FLAG M2 monoclonal antibody: Difference between pages

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(Created page with "'''Antigen:''' FLAG epitope '''Epitope:''' aa DYKDDDDK '''Host Species/Isotype:''' mouse monoclonal '''Antigen Species:''' synthetic peptide '''Applications:''' ...")
 
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'''Antigen:''' c-myc
'''Antigen:''' FLAG epitope
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6052813 myc]
'''Epitope:''' aa DYKDDDDK
'''Epitope:''' aa 408-439 of human c-myc
'''Host Species/Isotype:''' mouse monoclonal
'''Host Species/Isotype:''' mouse monoclonal
'''Antigen Species:''' human
'''Applications:''' IF
'''Antigen Species:''' synthetic peptide
 
'''Antibody Name/Catalog Number:''' [https://www.roche-applied-science.com/servlet/RCProductDisplay?storeId=10202&catalogId=10202&langId=-1&countryId=us&forCountryId=us&productId=3.7.2.12.10.1 anti-c-myc (clone 9E10) 1 667 203]
'''Applications:''' IP, IF, WB
 
'''Supplier:''' Roche
'''Antibody Name:''' F3165 [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=F3165|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC]
 
'''Comments:''' IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, 0.5 ug/ml use at 1:500 to 1:1000
'''Supplier:''' Sigma
 
'''Contributor:''' Zorn Lab
'''Comments:''' IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000
 
'''Additional Information:'''
'''Contributor:''' Zorn Lab and V Ponferrada
'''Additional Information:''' IF: MEMFA, 10% TCA fixatives work
'''Reference(s):'''
'''Reference(s):'''
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IP - immunoprecipitation,
IP - immunoprecipitation,
 
IF - immunofluorescence,
IF - immunofluorescence,
 
IHC - immunohistochemistry,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry
FCM - flow cytometry

Revision as of 09:04, 8 March 2011

Antigen: FLAG epitope

Epitope: aa DYKDDDDK

Host Species/Isotype: mouse monoclonal

Antigen Species: synthetic peptide

Applications: IP, IF, WB

Antibody Name: F3165 [1]

Supplier: Sigma

Comments: IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000

Contributor: Zorn Lab and V Ponferrada

Additional Information: IF: MEMFA, 10% TCA fixatives work

Reference(s):



applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

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