Anti-FLAG M2 monoclonal antibody and C-cadherin - cdh3: Difference between pages

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'''Antigen:''' FLAG epitope
'''Antigen:''' C-cadherin
'''Epitope:''' aa DYKDDDDK
'''Epitope:''' C-cadherin extracellular domain fused to GST
'''Host Species/Isotype:''' mouse monoclonal
'''Host Species/Isotype:''' mouse monoclonal
'''Antigen Species:''' synthetic peptide
'''Antigen Species:''' xenopus
'''Applications:''' IP, IF, WB
'''Applications:''' IF
'''Antibody Name:''' F3165 [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=F3165|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC]
'''Antibody Name:''' [http://dshb.biology.uiowa.edu/cadherin-C- 6B6]
'''Supplier:''' Sigma
'''Supplier:''' Developmental Studies Hybridoma Bank
'''Comments:''' IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000
'''Comments:''' IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300
'''Contributor:''' Zorn Lab and V Ponferrada
'''Contributor:''' Zorn Lab and V. Ponferrada
'''Additional Information:''' IF: MEMFA, 10% TCA fixatives work
'''Additional Information:''' MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work
'''Reference(s):'''
'''Reference(s):'''

Revision as of 12:54, 8 March 2011

Antigen: C-cadherin

Epitope: C-cadherin extracellular domain fused to GST

Host Species/Isotype: mouse monoclonal

Antigen Species: xenopus

Applications: IF

Antibody Name: 6B6

Supplier: Developmental Studies Hybridoma Bank

Comments: IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300

Contributor: Zorn Lab and V. Ponferrada

Additional Information: MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry