C-cadherin - cdh3 and PKC mu - prkd1: Difference between pages

Revision as of 12:05, 8 March 2011

Antigen: PKC mu

Epitope: mouse c-terminus

Host Species/Isotype: rabbit poly

Antigen Species: mouse

Applications: IF

Antibody Name: C-20 (sc-639) [1]

Supplier: Santa Cruz

Comments: IF: MEMFA 30 min @ RT, post-fix Dent's use at 1:300

Contributor: Zorn Lab

Additional Information:

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

@@ Line 1: / Line 1: @@
-'''Antigen:'''		C-cadherin
+'''Antigen:'''		PKC mu
-'''Xenbase Gene Page:'''		[http://www.xenbase.org/gene/showgene.do?method=display&geneId=482276 cdh3]
+'''Epitope:'''		mouse c-terminus
-'''Epitope:'''		C-cadherin extracellular domain fused to GST
+'''Host Species/Isotype:'''		rabbit poly
-'''Host Species/Isotype:'''		mouse monoclonal
+'''Antigen Species:'''		mouse
-'''Antigen Species:'''		xenopus
 '''Applications:'''		IF
-'''Antibody Name/Catalog Number:'''		[http://dshb.biology.uiowa.edu/cadherin-C- 6B6]
+'''Antibody Name:'''		C-20 (sc-639) [http://www.scbt.com/datasheet-639-pkc-mu-c-20-antibody.html]
-'''Supplier:'''		Developmental Studies Hybridoma Bank
+'''Supplier:'''		Santa Cruz
-'''Comments:'''		IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300
+'''Comments:'''		IF: MEMFA 30 min @ RT, post-fix Dent's use at 1:300
-'''Contributor:'''		Zorn Lab and V Ponferrada
+'''Contributor:'''		Zorn Lab
-'''Additional Information:'''		MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work
+'''Additional Information:'''
 '''Reference(s):'''