PKC mu - prkd1 and Histone H3, phosphorylated - h3f3a - mitotic cells - from upstate: Difference between pages

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(Created page with "'''Antigen:''' PKC mu '''Epitope:''' mouse c-terminus '''Host Species/Isotype:''' rabbit poly '''Antigen Species:''' mouse '''Applications:''' IF '''Antibody ...")
 
(Created page with "'''Antigen:''' phosporylated histone H3 (Ser10) '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a] '''Epitope:''' KLH-c...")
 
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'''Antigen:''' PKC mu
'''Antigen:''' phosporylated histone H3 (Ser10)
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a]
'''Epitope:''' mouse c-terminus
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
 
'''Host Species/Isotype:'''
 
'''Antigen Species:''' human
'''Host Species/Isotype:''' rabbit poly
'''Applications:''' IF, WB
 
'''Antigen Species:''' mouse
'''Antibody Name/Catalog Number:'''
 
'''Applications:''' IF
'''Supplier:''' [http://www.millipore.com/catalogue/item/06-570 Upstate/Millipore, Cat # 06-570]
 
'''Antibody Name:''' C-20 (sc-639) [http://www.scbt.com/datasheet-639-pkc-mu-c-20-antibody.html]
'''Comments:''' not sentitive to bleaching, works post in situ
 
'''Supplier:''' Santa Cruz
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Comments:''' IF: MEMFA 30 min @ RT, post-fix Dent's use at 1:300
'''Contributor:''' Zorn Lab
'''Additional Information:'''
'''Additional Information:'''
'''Reference(s):'''
'''Reference(s):'''
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IP - immunoprecipitation,
IP - immunoprecipitation,
 
IF - immunofluorescence,
IF - immunofluorescence,
 
IHC - immunohistochemistry,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry
FCM - flow cytometry

Revision as of 13:49, 8 March 2011

Antigen: phosporylated histone H3 (Ser10)

Xenbase Gene Page: h3f3a

Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Host Species/Isotype:

Antigen Species: human

Applications: IF, WB

Antibody Name/Catalog Number:

Supplier: Upstate/Millipore, Cat # 06-570

Comments: not sentitive to bleaching, works post in situ

Contributor: Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry