5A3 - somite and heart and P44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma: Difference between pages

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(Created page with "'''Antigen:''' dissected tadpole tissue '''Xenbase Gene Page:''' '''Epitope:''' '''Host Species/Isotype:''' mouse, IgG '''Antigen Species:''' Xenopus laevis '''Applications:...")
 
(Created page with "'''Antigen:''' phospho p44/42 MAPK '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479466 mapk1] '''Epitope:''' HTGFLTpEYpVAT '''H...")
 
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'''Antigen:''' dissected tadpole tissue
'''Antigen:''' phospho p44/42 MAPK


'''Xenbase Gene Page:'''
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479466 mapk1]
'''Epitope:''' HTGFLTpEYpVAT


'''Epitope:'''
'''Host Species/Isotype:''' mouse monoclonal


'''Host Species/Isotype:''' mouse, IgG
'''Antigen Species:'''
'''Applications:''' IF, WB


'''Antigen Species:''' Xenopus laevis
'''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N4=m9692&D7=0&D10=m9692&N1=S_ID&ST=RS&N25=0&F=PR monoclonal anti-MAP kinase, activated (diphosphorylated ERK1&2) M9692]


'''Applications:'''  
'''Supplier:''' sigma


'''Antibody Name/Catalog Number:''' 5A3
'''Comments:''' IF: MEMFA 1-2 hrs @ RT, post fix Dent's use at 1:500, WB: use at 1:5000 to 1:10,000


'''Supplier:''' EXRC
'''Contributor:''' Zorn Lab


'''Comments:'''Supplied as tissue culture supernatent
'''Additional Information:'''
'''Reference(s):'''
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,


'''Contributor:''' Developed by Liz Jones
IF - immunofluorescence,


[[File:5A3.jpg]]
IHC - immunohistochemistry,
EMSA - electromobility shift assay,


'''Additional Information:'''
ChIP - chromatin immunoprecipitation,


'''Reference(s):'''
FCM - flow cytometry

Latest revision as of 10:07, 9 March 2011

Antigen: phospho p44/42 MAPK

Xenbase Gene Page: mapk1

Epitope: HTGFLTpEYpVAT

Host Species/Isotype: mouse monoclonal

Antigen Species:

Applications: IF, WB

Antibody Name/Catalog Number: monoclonal anti-MAP kinase, activated (diphosphorylated ERK1&2) M9692

Supplier: sigma

Comments: IF: MEMFA 1-2 hrs @ RT, post fix Dent's use at 1:500, WB: use at 1:5000 to 1:10,000

Contributor: Zorn Lab

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry