P44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma (2) and P44/42 MAPK, ERK1/2 phosphorylated, alkaline phosphatase conjugate - mapk1 - from sigma: Difference between pages

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(Created page with "'''Antigen:''' phospho p44/42 MAPK '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479466 mapk1] '''Epitope:''' HTGFLTpEYpVAT '''H...")
 
(Created page with "'''Antigen:''' phospho p44/42 MAPK '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479466 mapk1] '''Epitope:''' HTGFLTpEYpVAT '''H...")
 
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'''Antigen Species:'''
'''Antigen Species:'''
'''Applications:''' WB
'''Applications:''' IF


'''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N4=m8159&D7=0&D10=m8159&N1=S_ID&ST=RS&N25=0&F=PR monoclonal anti-MAP kinase, activated (diphosphorylated ERK1&20 ascites fluid M8159]
'''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N4=A3713&D7=0&D10=A3713&N1=S_ID&ST=RS&N25=0&F=PR monoclonal anti-MAP kinase, activated (diphosphorylated ERK1&20 alkaline phosphatase conjugate A3713]


'''Supplier:''' sigma
'''Supplier:''' sigma


'''Comments:''' WB: use at 1:5000 to 1:10,000
'''Comments:''' IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, use @ 1:500


'''Contributor:''' Zorn Lab
'''Contributor:''' Zorn Lab

Latest revision as of 10:10, 9 March 2011

Antigen: phospho p44/42 MAPK

Xenbase Gene Page: mapk1

Epitope: HTGFLTpEYpVAT

Host Species/Isotype: mouse monoclonal

Antigen Species:

Applications: IF

Antibody Name/Catalog Number: monoclonal anti-MAP kinase, activated (diphosphorylated ERK1&20 alkaline phosphatase conjugate A3713

Supplier: sigma

Comments: IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, use @ 1:500

Contributor: Zorn Lab

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry