Alpha-tubulin - tuba4b - microtubules: Difference between revisions
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Revision as of 14:13, 4 February 2011
Antigen: microtubules from chick embryo brain
Epitope:
Host Species/Isotype: mouse IgG
Antigen Species: chicken
Applications:
Antibody Name: DM1A
Supplier: Sigma (cat#T9026)
Comments: fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
Contributor: Wallingford Lab
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
Additional Comments:
Available as: Ascites
Optimized conditions: We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.
Other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse