Alpha-tubulin - tuba4b - microtubules: Difference between revisions

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'''Antigen:''' microtubules from chick embryo brain
''Antigen:''' microtubules from chick embryo brain
'''Epitope:'''
'''Epitope:'''
'''Host Species/Isotype:''' mouse IgG
'''Host Species/Isotype:''' mouse IgG
'''Antigen Species:''' chicken
'''Antigen Species:''' chicken
'''Applications:'''
'''Applications:'''
'''Antibody Name:''' DM1A
'''Antibody Name:''' DM1A
'''Supplier:''' Sigma (cat#T9026)
'''Supplier:''' Sigma (cat#T9026) [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=T6199|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC]
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
'''Contributor:''' Wallingford Lab
'''Contributor:''' Wallingford Lab
 
'''Additional Information:''' other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
applications abbreviations:
WB - Western blotting,
'''Reference(s):'''
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
applications abbreviations:
 
Additional Comments:
WB - Western blotting,
'''Available as:''' Ascites
IP - immunoprecipitation,
 
'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.
IF - immunofluorescence,
 
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Revision as of 08:21, 8 March 2011

Antigen:' microtubules from chick embryo brain

Epitope:

Host Species/Isotype: mouse IgG

Antigen Species: chicken

Applications:

Antibody Name: DM1A

Supplier: Sigma (cat#T9026) [1]

Comments: fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ

Contributor: Wallingford Lab

Additional Information: other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse

Reference(s):



applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry