Alpha-tubulin - microtubules: Difference between revisions
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'''Antibody | '''Antigen:''' microtubules from chick embryo brain | ||
'''Epitope:''' | |||
'''Host Species/Isotype:''' mouse IgG | |||
'''Antigen Species:''' chicken | |||
'''Applications:''' | |||
'''Antibody Name:''' DM1A | |||
'''Supplier:''' Sigma (cat#T9026) | |||
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ | |||
'''Contributor:''' Wallingford Lab | |||
applications abbreviations: | |||
WB - Western blotting, | |||
IP - immunoprecipitation, | |||
IF - immunofluorescence, | |||
IHC - immunohistochemistry, | |||
EMSA - electromobility shift assay, | |||
ChIP - chromatin immunoprecipitation, | |||
FCM - flow cytometry | |||
Additional Comments: | |||
'''Available as:''' Ascites | '''Available as:''' Ascites | ||
'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours. | '''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours. | ||
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse | '''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse | ||
Latest revision as of 14:01, 4 February 2011
Antigen: microtubules from chick embryo brain
Epitope:
Host Species/Isotype: mouse IgG
Antigen Species: chicken
Applications:
Antibody Name: DM1A
Supplier: Sigma (cat#T9026)
Comments: fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
Contributor: Wallingford Lab
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
Additional Comments:
Available as: Ascites
Optimized conditions: We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.
Other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse