Phospho-histone H3 - h3f3a - mitotic cells - from upstate and Active b-catenin - ctnnb1: Difference between pages

Latest revision as of 08:44, 8 March 2011

Antigen: human b-catenin

Epitope: aa 36-44

Host Species/Isotype: mouse monoclonal IgG1 clone 8E7

Antigen Species: human

Applications: WB

Antibody Name: anti-ABC 05-665 [1]

Supplier: Upstate Cell Signaling Solutions/Millipore

Comments: WB: use at 1:1000

Contributor: Zorn Lab

Additional Information:

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

@@ Line 1: / Line 1: @@
-'''Antigen:'''		phosporylated histone H3 (Ser10)
+'''Antigen:'''		human b-catenin
-'''Epitope:'''		KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
+'''Epitope:'''		aa 36-44
-'''Host Species/Isotype:'''
+'''Host Species/Isotype:'''		mouse monoclonal IgG1 clone 8E7
 '''Antigen Species:'''		human
-'''Applications:'''		IF, WB
+'''Applications:'''		WB
-'''Antibody Name:'''
+'''Antibody Name:'''		anti-ABC 05-665 [http://www.millipore.com/catalogue/item/05-665]
-'''Supplier:'''		Upstate/Millipore, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]
+'''Supplier:'''		Upstate Cell Signaling Solutions/Millipore
-'''Comments:'''		not sentitive to bleaching, works post in situ
+'''Comments:'''		WB: use at 1:1000
-'''Contributor:'''		Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
+'''Contributor:'''		Zorn Lab
 '''Additional Information:'''