C-myc - myc - from sigma and C-myc - myc - from roche: Difference between pages
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'''Antigen:''' | '''Antigen:''' E-cadherin | ||
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId= | '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=12531593 cdh1] | ||
'''Epitope:''' | '''Epitope:''' 140 kDa xenopus E-cadherin | ||
'''Host Species/Isotype:''' mouse monoclonal | '''Host Species/Isotype:''' mouse monoclonal IgG2b | ||
'''Antigen Species:''' | '''Antigen Species:''' xenopus | ||
'''Applications:''' IF | '''Applications:''' IF | ||
'''Antibody Name/Catalog Number:''' [http:// | '''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/cadherin-E- 5D3] | ||
'''Supplier:''' | '''Supplier:''' Developmental Systems Hybridoma Bank | ||
'''Comments:''' IF: | '''Comments:''' IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300 | ||
'''Contributor:''' Zorn Lab | '''Contributor:''' Zorn Lab and V Ponferrada | ||
'''Additional Information:''' | '''Additional Information:''' MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work | ||
'''Reference(s):''' | '''Reference(s):''' |
Revision as of 15:51, 8 March 2011
Antigen: E-cadherin
Xenbase Gene Page: cdh1
Epitope: 140 kDa xenopus E-cadherin
Host Species/Isotype: mouse monoclonal IgG2b
Antigen Species: xenopus
Applications: IF
Antibody Name/Catalog Number: 5D3
Supplier: Developmental Systems Hybridoma Bank
Comments: IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
Contributor: Zorn Lab and V Ponferrada
Additional Information: MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry