C-myc - myc - from sigma and C-myc - myc - from roche: Difference between pages

From XenWiki
(Difference between pages)
Jump to navigation Jump to search
No edit summary
 
No edit summary
 
Line 1: Line 1:
'''Antigen:''' c-myc
'''Antigen:''' E-cadherin
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6052813 myc]
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=12531593 cdh1]
'''Epitope:''' aa 408-439 synthetic peptide
'''Epitope:''' 140 kDa xenopus E-cadherin
'''Host Species/Isotype:''' mouse monoclonal
'''Host Species/Isotype:''' mouse monoclonal IgG2b
'''Antigen Species:''' human
'''Antigen Species:''' xenopus
'''Applications:''' IF, WB
'''Applications:''' IF
'''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/ProductDetail.do?F=SPEC&N4=M4439|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&cm_sp=Baynote-_-CountryCode~US|WebLang~-1|-_-m4439 anti-c-myc clone 9E10 M4439]
'''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/cadherin-E- 5D3]
'''Supplier:''' Sigma
'''Supplier:''' Developmental Systems Hybridoma Bank
'''Comments:''' IF: MEMFA 1-2 hrs @ RT, post fix Dent's, WB: use at 1:1000 to 1:10,000
'''Comments:''' IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
'''Contributor:''' Zorn Lab
'''Contributor:''' Zorn Lab and V Ponferrada
'''Additional Information:''' detects myc-tagged overexpressed proteins well
'''Additional Information:''' MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
'''Reference(s):'''
'''Reference(s):'''

Revision as of 15:51, 8 March 2011

Antigen: E-cadherin

Xenbase Gene Page: cdh1

Epitope: 140 kDa xenopus E-cadherin

Host Species/Isotype: mouse monoclonal IgG2b

Antigen Species: xenopus

Applications: IF

Antibody Name/Catalog Number: 5D3

Supplier: Developmental Systems Hybridoma Bank

Comments: IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300

Contributor: Zorn Lab and V Ponferrada

Additional Information: MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry