Straight distal segment and Histone H3, phosphorylated - h3f3a - mitotic cells - from upstate: Difference between pages

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#REDIRECT [[XB-ANAT-325]]
'''Antigen:''' phosporylated histone H3 (Ser10)
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a]
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
 
'''Host Species/Isotype:'''
 
'''Antigen Species:''' human
'''Applications:''' IF, WB
 
'''Antibody Name/Catalog Number:''' [http://www.millipore.com/catalogue/item/06-570 Cat # 06-570]
 
'''Supplier:''' Upstate/Millipore
 
'''Comments:''' not sentitive to bleaching, works post in situ
 
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Additional Information:'''
[[File:Ph3.jpg|option|400px]]
 
 
A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
 
IF - immunofluorescence,
 
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry

Revision as of 09:04, 20 April 2011

Antigen: phosporylated histone H3 (Ser10)

Xenbase Gene Page: h3f3a

Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Host Species/Isotype:

Antigen Species: human

Applications: IF, WB

Antibody Name/Catalog Number: Cat # 06-570

Supplier: Upstate/Millipore

Comments: not sentitive to bleaching, works post in situ

Contributor: Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]

Additional Information:


option


A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry