Xenopus development stages and Histone H3, phosphorylated - h3f3a - mitotic cells - from upstate: Difference between pages

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*Embryonic Stage
'''Antigen:''' phosporylated histone H3 (Ser10)
**[[Stage 1]]
**Cleavage
***[[Stage 2]]
***[[Stage 3]]
***[[Stage 4]]
***[[Stage 5]]
***[[Stage 6]]
**Blastula
***[[Stage 7]]
***[[Stage 8]]
***[[Stage 9]]
**Gastrula
***[[Stage 10]]
***[[Stage 11]]
***[[Stage 12]]
**Neurula
***[[Stage 13]]
***[[Stage 14]]
***[[Stage 15]]
***[[Stage 16]]
***[[Stage 17]]
***[[Stage 18]]
***[[Stage 19]]
***[[Stage 20]]
***[[Stage 21]]
**Tailbud
***Early tailbud
****[[Stage 22]]
****[[Stage 23]]
****[[Stage 24]]
****[[Stage 25]]
****[[Stage 26]]
****[[Stage 27]]
****[[Stage 28]]
***Late tailbud
****[[Stage 29/30|Stage 29 and 30]]
****[[Stage 31]]
****[[Stage 32]]
****[[Stage 33/34|Stage 33 and 34]]
****[[Stage 35/36|Stage 35 and 36]]
****[[Stage 37/38|Stage 37 and 38]]
****[[Stage 39]]
****[[Stage 40]]
****[[Stage 41]]
****[[Stage 42]]
****[[Stage 43]]
****[[Stage 44]]
**Tadpole
***Premetamorphosis
****[[Stage 45]]
****[[Stage 46]]
****[[Stage 47]]
****[[Stage 48]]
****[[Stage 49]]
****[[Stage 50]]


'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a]
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3


Digitized images and developmental data from Nieuwkoop and Faber (1994) Normal Table of Xenopus laevis (Daudin). Garland Publishing Inc, New York.  Copyright © 1994 Pieter D. Nieuwkoop and J. Faber.
'''Host Species/Isotype:'''


Digital photographs of Xenopus embryos used with permission by Barbra Lom, work performed by William Graham, and Ian Willoughby. All rights reserved. No portion of them can be used without prior expressed permission [http://www.bio.davidson.edu/people/balom/StagingTable/Preface.html].
'''Antigen Species:''' human
'''Applications:''' IF, WB
 
'''Antibody Name/Catalog Number:''' [http://www.millipore.com/catalogue/item/06-570 Cat # 06-570]
 
'''Supplier:''' Upstate/Millipore
 
'''Comments:''' not sentitive to bleaching, works post in situ
 
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Additional Information:'''
[[File:Ph3.jpg|option|400px]]
 
 
A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
 
IF - immunofluorescence,
 
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry

Revision as of 09:04, 20 April 2011

Antigen: phosporylated histone H3 (Ser10)

Xenbase Gene Page: h3f3a

Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Host Species/Isotype:

Antigen Species: human

Applications: IF, WB

Antibody Name/Catalog Number: Cat # 06-570

Supplier: Upstate/Millipore

Comments: not sentitive to bleaching, works post in situ

Contributor: Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]

Additional Information:


option


A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

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