PKC mu - "gene name" and Histone H3, phosphorylated - h3f3a - mitotic cells - from upstate: Difference between pages
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'''Antigen:''' | '''Antigen:''' phosporylated histone H3 (Ser10) | ||
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a] | |||
'''Epitope:''' | '''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3 | ||
'''Host Species/Isotype:''' | |||
'''Antigen Species:''' human | |||
'''Applications:''' IF, WB | |||
'''Antibody Name/Catalog Number:''' [http://www.millipore.com/catalogue/item/06-570 Cat # 06-570] | |||
'''Applications:''' IF | '''Supplier:''' Upstate/Millipore | ||
'''Antibody Name:''' | '''Comments:''' not sentitive to bleaching, works post in situ | ||
'''Supplier:''' | '''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731] | ||
'''Comments:''' | |||
'''Contributor:''' | |||
'''Additional Information:''' | '''Additional Information:''' | ||
[[File:Ph3.jpg|option|400px]] | |||
A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase | |||
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IP - immunoprecipitation, | IP - immunoprecipitation, | ||
IF - immunofluorescence, | IF - immunofluorescence, | ||
IHC - immunohistochemistry, | IHC - immunohistochemistry, | ||
EMSA - electromobility shift assay, | EMSA - electromobility shift assay, | ||
ChIP - chromatin immunoprecipitation, | ChIP - chromatin immunoprecipitation, | ||
FCM - flow cytometry | FCM - flow cytometry | ||
Revision as of 09:04, 20 April 2011
Antigen: phosporylated histone H3 (Ser10)
Xenbase Gene Page: h3f3a
Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
Host Species/Isotype:
Antigen Species: human
Applications: IF, WB
Antibody Name/Catalog Number: Cat # 06-570
Supplier: Upstate/Millipore
Comments: not sentitive to bleaching, works post in situ
Contributor: Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]
Additional Information:
A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry