Collagen type II - col2a1 - ECM broad and Recipe: Difference between pages

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(Created page with "10 mm HEPES 60 mm NaCl 0.67 mm KCl 0.34 mm Ca(NO3)2 0.83 mm MgSO4 Adjust to pH 7.4. * © 2011 Cold Spring Harbor Laboratory Press")
 
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'''Antigen:''' chicken type II collagen
10 mm HEPES
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6258352 col2a1]
60 mm NaCl
 
'''Epitope:'''
0.67 mm KCl
 
'''Host Species/Isotype:''' mouse monoclonal IgG2a
0.34 mm Ca(NO3)2
 
'''Antigen Species:''' chicken
0.83 mm MgSO4
 
'''Applications:''' IF
Adjust to pH 7.4.
 
'''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/collagen-type-II CIIC1]
    * © 2011 Cold Spring Harbor Laboratory Press
'''Supplier:''' Developmental Studies Hybridoma Bank
'''Comments:''' works for IF but not well, fixative: Dent's
'''Contributor:''' Sandra Kolker and Dan Weeks
'''Additional Information:'''
'''Reference(s):'''
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Latest revision as of 13:09, 20 October 2011

10 mm HEPES

60 mm NaCl

0.67 mm KCl

0.34 mm Ca(NO3)2

0.83 mm MgSO4

Adjust to pH 7.4. 

   * © 2011 Cold Spring Harbor Laboratory Press
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