Phospho-histone H3 - h3f3a - mitotic cells - from upstate and Recipe: Difference between pages

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(Created page with "'''Antigen:''' phosporylated histone H3 (Ser10) '''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3 '''Host Sp...")
 
(Created page with "10 mm HEPES 60 mm NaCl 0.67 mm KCl 0.34 mm Ca(NO3)2 0.83 mm MgSO4 Adjust to pH 7.4. * © 2011 Cold Spring Harbor Laboratory Press")
 
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'''Antigen:''' phosporylated histone H3 (Ser10)
10 mm HEPES
 
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
60 mm NaCl
 
'''Host Species/Isotype:'''
0.67 mm KCl
 
'''Antigen Species:''' human
0.34 mm Ca(NO3)2
 
'''Applications:''' IF, WB
0.83 mm MgSO4
 
'''Antibody Name:'''
Adjust to pH 7.4.
 
'''Supplier:''' Upstate/Millipore, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]
    * © 2011 Cold Spring Harbor Laboratory Press
'''Comments:''' not sentitive to bleaching, works post in situ
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Additional Information:'''
'''Reference(s):'''
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Latest revision as of 13:09, 20 October 2011

10 mm HEPES

60 mm NaCl

0.67 mm KCl

0.34 mm Ca(NO3)2

0.83 mm MgSO4

Adjust to pH 7.4. 

   * © 2011 Cold Spring Harbor Laboratory Press
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