Active b-catenin - ctnnb1 and Recipe: Difference between pages

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(Created page with "'''Antigen:''' human b-catenin '''Epitope:''' aa 36-44 '''Host Species/Isotype:''' mouse monoclonal IgG1 clone 8E7 '''Antigen Species:''' human '''Applications:''...")
 
(Created page with "10 mm HEPES 60 mm NaCl 0.67 mm KCl 0.34 mm Ca(NO3)2 0.83 mm MgSO4 Adjust to pH 7.4. * © 2011 Cold Spring Harbor Laboratory Press")
 
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'''Antigen:''' human b-catenin
10 mm HEPES
 
'''Epitope:''' aa 36-44
60 mm NaCl
 
'''Host Species/Isotype:''' mouse monoclonal IgG1 clone 8E7
0.67 mm KCl
 
'''Antigen Species:''' human
0.34 mm Ca(NO3)2
 
'''Applications:''' WB
0.83 mm MgSO4
 
'''Antibody Name:''' anti-ABC 05-665 [http://www.millipore.com/catalogue/item/05-665]
Adjust to pH 7.4.
 
'''Supplier:''' Upstate Cell Signaling Solutions/Millipore
    * © 2011 Cold Spring Harbor Laboratory Press
'''Comments:''' WB: use at 1:1000
'''Contributor:''' Zorn Lab
'''Additional Information:'''
'''Reference(s):'''
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Latest revision as of 13:09, 20 October 2011

10 mm HEPES

60 mm NaCl

0.67 mm KCl

0.34 mm Ca(NO3)2

0.83 mm MgSO4

Adjust to pH 7.4. 

   * © 2011 Cold Spring Harbor Laboratory Press
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