Alpha-catenin - ctnna1 and Recipe: Difference between pages

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(Created page with "10 mm HEPES 60 mm NaCl 0.67 mm KCl 0.34 mm Ca(NO3)2 0.83 mm MgSO4 Adjust to pH 7.4. * © 2011 Cold Spring Harbor Laboratory Press")
 
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'''Antigen:''' human/mouse alpha-catenin
10 mm HEPES
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479598 ctnna1]
60 mm NaCl
 
'''Epitope:''' aa 890-901
0.67 mm KCl
 
'''Host Species/Isotype:''' rabbit
0.34 mm Ca(NO3)2
 
'''Antigen Species:''' human
0.83 mm MgSO4
 
'''Applications:''' IF, WB
Adjust to pH 7.4.
 
'''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=C2081|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC anti-a-catenin C 2081]
    * © 2011 Cold Spring Harbor Laboratory Press
'''Supplier:''' Sigma
'''Comments:''' IF: MEMFA 1-2 hr @ RT, post-fix in Dent's, use at 1:300 WB: use at 1:5000
'''Contributor:''' Zorn Lab
'''Additional Information:'''
'''Reference(s):'''
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Latest revision as of 13:09, 20 October 2011

10 mm HEPES

60 mm NaCl

0.67 mm KCl

0.34 mm Ca(NO3)2

0.83 mm MgSO4

Adjust to pH 7.4. 

   * © 2011 Cold Spring Harbor Laboratory Press
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