PKC mu - "gene name" and Recipe: Difference between pages

Latest revision as of 13:09, 20 October 2011

10 mm HEPES

60 mm NaCl

0.67 mm KCl

0.34 mm Ca(NO3)2

0.83 mm MgSO4

Adjust to pH 7.4.

   * © 2011 Cold Spring Harbor Laboratory Press

@@ Line 1: / Line 1: @@
-'''Antigen:'''		PKC mu
+mm HEPES
-'''Epitope:'''		mouse c-terminus
+mm NaCl
-'''Host Species/Isotype:'''		rabbit poly
+.67 mm KCl
-'''Antigen Species:'''		mouse
+.34 mm Ca(NO3)2
-'''Applications:'''		IF
+.83 mm MgSO4
-'''Antibody Name:'''		C-20 (sc-639) [http://www.scbt.com/datasheet-639-pkc-mu-c-20-antibody.html]
+Adjust to pH 7.4.
-'''Supplier:'''		Santa Cruz
+    * © 2011 Cold Spring Harbor Laboratory Press
-'''Comments:'''		IF: MEMFA 30 min @ RT, post-fix Dent's use at 1:300
-'''Contributor:'''		Zorn Lab
-'''Additional Information:'''
-'''Reference(s):'''
-applications abbreviations:
-WB - Western blotting,
-IP - immunoprecipitation,
-IF - immunofluorescence,
-IHC - immunohistochemistry,
-EMSA - electromobility shift assay,
-ChIP - chromatin immunoprecipitation,
-FCM - flow cytometry