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| '''Antigen:''' E-cadherin
| | 10 mm HEPES |
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| '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=12531593 cdh1]
| | 60 mm NaCl |
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| '''Epitope:''' 140 kDa xenopus E-cadherin
| | 0.67 mm KCl |
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| '''Host Species/Isotype:''' mouse monoclonal IgG2b
| | 0.34 mm Ca(NO3)2 |
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| '''Antigen Species:''' xenopus
| | 0.83 mm MgSO4 |
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| '''Applications:''' IF
| | Adjust to pH 7.4. |
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| '''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/cadherin-E- 5D3]
| | * © 2011 Cold Spring Harbor Laboratory Press |
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| '''Supplier:''' Developmental Systems Hybridoma Bank
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| '''Comments:''' IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
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| '''Contributor:''' Zorn Lab and V Ponferrada
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| '''Additional Information:''' MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
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| '''Reference(s):'''
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| applications abbreviations:
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| WB - Western blotting,
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| IP - immunoprecipitation,
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| IF - immunofluorescence,
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| IHC - immunohistochemistry,
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| EMSA - electromobility shift assay,
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| ChIP - chromatin immunoprecipitation,
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| FCM - flow cytometry
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Latest revision as of 13:09, 20 October 2011
10 mm HEPES
60 mm NaCl
0.67 mm KCl
0.34 mm Ca(NO3)2
0.83 mm MgSO4
Adjust to pH 7.4.
* © 2011 Cold Spring Harbor Laboratory Press