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| Line 1: |
Line 1: |
| '''Antigen:''' phosporylated histone H3 (Ser10)
| | 10 mm HEPES |
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| '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a]
| | 60 mm NaCl |
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| '''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
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| '''Host Species/Isotype:'''
| | 0.67 mm KCl |
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| '''Antigen Species:''' human
| | 0.34 mm Ca(NO3)2 |
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| '''Applications:''' IF, WB
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| '''Antibody Name/Catalog Number:''' [http://www.millipore.com/catalogue/item/06-570 Cat # 06-570]
| | 0.83 mm MgSO4 |
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| '''Supplier:''' Upstate/Millipore
| | Adjust to pH 7.4. |
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| '''Comments:''' not sentitive to bleaching, works post in situ
| | * © 2011 Cold Spring Harbor Laboratory Press |
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| '''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
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| '''Additional Information:'''
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| [[File:Ph3.jpg|option|400px]]
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| A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase
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| applications abbreviations:
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| WB - Western blotting,
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| IP - immunoprecipitation,
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| IF - immunofluorescence,
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| IHC - immunohistochemistry,
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| EMSA - electromobility shift assay,
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| ChIP - chromatin immunoprecipitation,
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| FCM - flow cytometry
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